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. 2019 Jun 13;20(1):107.
doi: 10.1186/s12881-019-0846-3.

Association of KCNJ11 rs5219 gene polymorphism with type 2 diabetes mellitus in a population of Syria: a case-control study

Osama Makhzoom  1 , Younes Kabalan  2 , Faizeh Al-Quobaili  3
Affiliations

Association of KCNJ11 rs5219 gene polymorphism with type 2 diabetes mellitus in a population of Syria: a case-control study

Osama Makhzoom et al. BMC Med Genet. .

Abstract

Background: Type 2 diabetes mellitus is believed to be a polygenic disorder that develops as a result of a complex interaction between multiple genes and environmental factors. KCNJ11 gene encodes a Kir6.2 protein which forms the inner section of the potassium channels in pancreatic beta cells. Several studies found that KCNJ11 polymorphism increases T2DM risk. Our study aimed to investigate the association between rs5219 polymorphism of the KCNJ11 gene and T2DM in Syrian patients.

Methods: This case-control study involved 75 T2DM patients and 63 healthy controls. The KCNJ11 rs5219 polymorphism was genotyped by Restriction Fragment Length Polymorphism (RFLP).

Results: The frequency of the risk allele K was similar between the two groups (38.7% vs. 38.1%, P = 0.132). The frequency of the KK genotype was higher among the patients' group (16% vs. 4.8%), and the frequency of the EK genotype was higher among the control group (45.3% vs. 66.6%); however, the differences were statistically insignificant. The KK genotype was significantly associated with T2DM in the recessive model with an OR of 3.81 (95% CI 1.024-14.17, P = 0.035).

Conclusions: This study showed that rs5219 polymorphism of the KCNJ11 gene is an important risk factor for type 2 diabetes mellitus in a sample of the Syrian population.

Keywords: Beta cells; KCNJ11; Polymorphism; Potassium channels; RFLP; Syria; Type 2 diabetes; rs5219.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
The product of the PCR amplification of KCNJ11- rs5219. Samples were electrophoresed on a 2% agarose gel. Lane M: 100 bp ladder, Lane (1,2,3,4,5,6): PCR products (210 bp), Lane 7: negative control
Fig. 2
Fig. 2
Digestion products for KCNJ11- rs5219 by BanII enzyme. Samples were electrophoresed on a 2% agarose gel. Lane M: 100 bp ladder, Lane (1,4): homozygote EE, Lane (2,5): heterozygote EK, Lane (3,6): homozygote KK
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References

    1. Cho NH, Shaw JE, Karuranga S, Huang Y, da Rocha Fernandes JD, Ohlrogge AW, Malanda B. IDF diabetes atlas: global estimates of diabetes prevalence for 2017 and projections for 2045. Diabetes Res Clin Pract. 2018;138:271–281. doi: 10.1016/j.diabres.2018.02.023. - DOI - PubMed
    1. National Diabetes Statistics Report, 2017 Estimates of Diabetes and Its Burden in the United States2017. https://www.cdc.gov/diabetes/pdfs/data/statistics/national-diabetes-stat.... Accessed Feb 2018.
    1. World Health Organization – Diabetes country profiles, Syrian Arab Republic, 2016. https://www.who.int/diabetes/country-profiles/syr_en.pdf?ua=1.
    1. Kwak SH, Park KS. Recent progress in genetic and epigenetic research on type 2 diabetes. Exp Mol Med. 2016;48(3):e220. doi: 10.1038/emm.2016.7. - DOI - PMC - PubMed
    1. Ali O. Genetics of type 2 diabetes. World J Diabetes. 2013;4(4):114–123. doi: 10.4239/wjd.v4.i4.114. - DOI - PMC - PubMed

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