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. 2019 Jun 13;19(1):575.
doi: 10.1186/s12885-019-5794-y.

Treatment with somatostatin analogs induces differentially expressed let-7c-5p and mir-3137 in small intestine neuroendocrine tumors

Florian Bösch  1   2 , Alexandr V Bazhin  3 , Sabine Heublein  4 , Katharina Brüwer  3   5 , Thomas Knösel  6   5 , Florian P Reiter  7   5 , Christoph J Auernhammer  8   5 , Markus O Guba  3   5 , Christine Spitzweg  8   5 , Jens Werner  3   5 , Martin K Angele  3   5
Affiliations

Treatment with somatostatin analogs induces differentially expressed let-7c-5p and mir-3137 in small intestine neuroendocrine tumors

Florian Bösch et al. BMC Cancer. .

Abstract

Background: Distant metastases frequently occur in gastroenteropancreatic neuroendocrine tumors. If hepatic surgery is not feasible, patients are treated with somatostatin analogs. However, the underlying mechanisms of action of this treatment remain to be defined. The aim of the present study was to analyze the micro-RNA expression profile inter-individually before and after the treatment with somatostatin analogs.

Material and methods: Tumor specimens of all included patients (n = 8) before and after the onset of a therapy with somatostatin analogs were analyzed and a micro-RNA expression profile (754 micro-RNAs) of each probe was generated. This analysis in an intra-individual setting was selected to avoid bias from inter-individual differences. The micro-RNA expression profiles were validated by qPCR. Patients with any other systemic treatment were excluded from the present study.

Results: Eight patients were included in the present study of which all had neuroendocrine tumors of the small intestine with diffuse hepatic metastases. Grouped analyses revealed that 15 micro-RNAs were differentially expressed (3 up- and 12 downregulated) after the exposure to somatostatin analogs. Additionally, let-7c-5p and mir-3137 are concordantly regulated in the inter-individually analysis.

Conclusions: This is the first study analyzing the individual micro-RNA expression profile before and after a therapy with somatostatin analogs. Data from this study reveal that somatostatin analogs may in part exert their beneficial effects through an alteration in the micro-RNA expression profile.

Keywords: Intra-individual; Neuroendocrine tumor; Somatostatin; microRNA.

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Conflict of interest statement

C J A has received research contracts (Ipsen, Novartis), lecture honorarium (Ipsen, Novartis, Pfizer, Falk) and advisory board honorarium (Novartis). The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
The heat map of the micro-RNA profiles analyzed
Fig. 2
Fig. 2
a Expression levels of the uniformly regulated micro-RNAs. let-7c-5p could be analyzed in patients 2–8 and was concordantly regulated. b mir-3137 could be analyzed in patients 2 and 4–8 and was concordantly regulated
See this image and copyright information in PMC

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