Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Oct;43(10):1743-8.

[Identification of the functional groups of yeast thiamine pyrophosphokinase]

[Article in Russian]
A I Voskoboev, V P Grinevich
  • PMID: 31197

[Identification of the functional groups of yeast thiamine pyrophosphokinase]

[Article in Russian]
A I Voskoboev et al. Biokhimiia. 1978 Oct.

Abstract

The content of free sulfhydril groups in yeast thiamine pyrophosphokinase (EC 2.7.6.2) was studied. Their blocking was found not to affect considerably the enzyme activity. N-bromsuccinimide developes the inhibitory effect only if taken in excessive concentrations, which indicates that tryptophane has no key position for the enzyme-substrate complex formation. On account of high speed of photoinactivation with Rose bengale and methilene blue, sigmoid dependence of activity loss on pH under irradiation, characteristic narrowing of the modified enzyme absorption spectrum, it is suggested that imidazole residue of the histidine is one of the functional groups of thiamine pyrophosphokinase.

PubMed Disclaimer

Publication types