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. 2019:2016:81-85.
doi: 10.1007/978-1-4939-9570-7_8.

Methods for Transposon Mutagenesis in Proteus mirabilis

Philip N Rather  1   2
Affiliations

Methods for Transposon Mutagenesis in Proteus mirabilis

Philip N Rather. Methods Mol Biol. 2019.

Abstract

Several methods for transposon mutagenesis have been employed for use in P. mirabilis. The first method involves the use of mini-Tn5 derivatives, which are delivered by conjugation of a suicide plasmid containing this transposon, followed by transposition into the chromosome. A second method is the use of preformed transposon/transposase complexes (transposomes), which are introduced into P. mirabilis cells by electroporation. Each of these methods will be discussed along with the advantages and disadvantages of each.

Keywords: Mini-Tn5; Proteus; Swarming; Transposome; Transposon mutagenesis; Virulence.

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