Construction of DNA Barcode-Tagged Salmonella Strains

Abstract

This chapter provides a detailed protocol for construction of DNA barcode-tagged isogenic strains of Salmonella. The protocol is illustrated with S. Enteritidis in this chapter. However, this protocol should be widely applicable to other Salmonella serotypes. A series of the DNA barcode-tagged strains thus constructed can be used in combination with next generation sequencing or quantitative PCR to study the population dynamics of the bacterial pathogen during infection within the host or transmission within a population of the host in a quantitative manner.

Keywords: DNA barcode-tagging; Next generation sequencing; Overlapping extension PCR; Population dynamics; Red recombination system; Salmonella.