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. 2019 May;8(5):1567-1570.
doi: 10.4103/jfmpc.jfmpc_136_19.

Toll like receptors (TLRs) in response to human gut microbiota of Indian obese and lean individuals

Tej Bahadur  1 , Rama Chaudhry  1 , Vishwa Deepak Bamola  1 , Sonu K Agrawal  1 , Pawan Malhotra  2 , Alka M Chutani  3 , Bijay R Mirdha  1 , Bimal K Das  1 , Rahul K Sharma  4 , Chandan K Thakur  1
Affiliations

Toll like receptors (TLRs) in response to human gut microbiota of Indian obese and lean individuals

Tej Bahadur et al. J Family Med Prim Care. 2019 May.

Abstract

Background: The rising incidence of obesity is one of the most serious public health issues in the developed as well as in developing countries like India. Obesity and overweight are most important risk factors for many chronic diseases, including cardiovascular diseases, diabetes and cancer. In this study the body mass index (BMI) cut off was taken as 18.5-22.9 kg/m2 for normal, 23.0-24.9 kg/m2 for Overweight and >25 kg/m2 for obese as per WHO recommendation for Asian Indians, which is different for developed and developing countries. Role of gut microbiota mediated immune response in the development of obesity has been studied but the literature on Indian population are lacking. Therefore, a study was conducted to determine Toll like receptors (TLRs) in response to human gut microbiota of Indian obese and lean individuals using viable colonocytes in a Non invasive technique and Flowcytometry.

Methods: A total of 20 healthy volunteer (10 obese and 10 lean) were enrolled in the study as per inclusion and exclusion criteria. Viable colonocytes were isolated from fecal samples using a Non invasive technique (SCSR Method). Toll like receptors (TLRs) and immunoglobulin (IgA &IgG) receptor concentration were measured by standard Flowcytometry methods using specific fluorochrome conjugated antibodies.

Results: Average TLR2 receptor concentration was significantly higher in obese (6.35 %) as compared to lean (2.9 %) (P = 0.01). TLR4 receptor concentration was 1.4 % in obese and 1.65 % in lean although the difference was not statistically significant (P = 0.59). IgA & IgG receptor concentration was 49.6 % & 11.2 % in the obese and 67.15 % & 8.05 % in the lean respectively but the differences among both the group were not statistically significant.

Conclusion: The results of the present study will be helpful for physicians and researchers to find some biomarkers which can determine predisposition of the obesity in Indian population and helps to use alternative therapeutics such as probiotics to maintain gut homeostasis and immune modulation to prevent obesity.

Keywords: Colonocytes; flowcytomety; lean; obese; toll-like receptors.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
The pictorial presentation of isolation of colonocytes from stool sample
Figure 2
Figure 2
Dot plots of flow cytometry demonstrating the expressions of IgA, IgG, TLR2, and TLR4. Relative expressions of markers were analyzed on the basis of their pattern in unstained tube. Figure a and a1): Forward scatter (FSC) and side scatter (SSC) pattern of the cells. Figure b, b1; c, c1; d, d1 and e, e1): Unstained and stained tubes for IgA, IgG, TLR2, and TLR4 expression, respectively
See this image and copyright information in PMC

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