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. 2019 Sep;12(5):845-848.
doi: 10.1111/1751-7915.13443. Epub 2019 Jun 14.

Pseudomonas putida KT2440 is HV1 certified, not GRAS

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Pseudomonas putida KT2440 is HV1 certified, not GRAS

Linde F C Kampers et al. Microb Biotechnol. 2019 Sep.

Abstract

Pseudomonas putida is rapidly becoming a workhorse for industrial production due to its metabolic versatility, genetic accessibility and stress-resistance properties. The P. putida strain KT2440 is often described as Generally Regarded as Safe, or GRAS, indicating the strain is safe to use as food additive. This description is incorrect. P. putida KT2440 is classified by the FDA as HV1 certified, indicating it is safe to use in a P1 or ML1 environment.

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Conflict of interest statement

None declared.

References

    1. Aparicio, T. , de Lorenzo, V. and Martínez‐García, E. (2018) CRISPR/Cas9‐based counterselection boosts recombineering efficiency in Pseudomonas putida . Biotechnol J 13: 1700161. - PubMed
    1. Arnfinnsdottir, N.B. , Bjørkøy, A.V. , Lale, R. , and Sletmoen, M. (2016) Heterogeneity in GFP expression in isogenic populations of P. putida KT2440 investigated using flow cytometry and bacterial microarrays. RSC Adv. 6: 36198–36206.
    1. Bagdasarian, M. , Lurz, R. , Rückert, B. , Franklin, F.C.H. , Bagdasarian, M.M. , Frey, J. , and Timmis, K.N. (1981) Specific‐purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010‐derived vectors, and a host‐vector system for gene cloning in Pseudomonas . Gene 16: 237–247. - PubMed
    1. Baritugo, K.‐A. , Kim, H.T. , Rhie, M.N. , Jo, S.Y. , Khang, T.U. , Kang, K.H. , et al (2018) Construction of a vitreoscilla hemoglobin promoter‐based tunable expression system for Corynebacterium glutamicum . Catalysts 8: 561.
    1. Belda, E. , van Heck, R.G.A. , José Lopez‐Sanchez, M. , Cruveiller, S. , Barbe, V. , Fraser, C. , et al (2016) The revisited genome of Pseudomonas putida KT2440 enlightens its value as a robust metabolic chassis. Environ Microbiol 18: 3403–3424. - PubMed

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