Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jun 13;10(2):26.
doi: 10.3390/jfb10020026.

Formulation of Antimicrobial Tobramycin Loaded PLGA Nanoparticles via Complexation with AOT

Marcus Hill  1 , Richard N Cunningham  2 , Rania M Hathout  3 , Christopher Johnston  4 , John G Hardy  5   6 , Marie E Migaud  7   8
Affiliations

Formulation of Antimicrobial Tobramycin Loaded PLGA Nanoparticles via Complexation with AOT

Marcus Hill et al. J Funct Biomater. .

Abstract

Tobramycin is a potent antimicrobial aminoglycoside and its effective delivery by encapsulation within nanoparticle carriers could increase its activity against infections through a combination of sustained release and enhanced uptake. Effective antimicrobial therapy against a clinically relevant model bacteria (Pseudomonas aeruginosa) requires sufficient levels of therapeutic drug to maintain a drug concentration above the microbial inhibitory concentration (MIC) of the bacteria. Previous studies have shown that loading of aminoglycoside drugs in poly(lactic-co-glycolic) acid (PLGA)-based delivery systems is generally poor due to weak interactions between the drug and the polymer. The formation of complexes of tobramycin with dioctylsulfosuccinate (AOT) allows the effective loading of the drug in PLGA-nanoparticles and such nanoparticles can effectively deliver the antimicrobial aminoglycoside with retention of tobramycin antibacterial function.

Keywords: antimicrobial; biomedical applications; colloids; drug delivery systems; nanoparticles.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
Chemical structures investigated in this report. (A) Tobramycin; (B) Fluorescent tobramycin derivative; (C) dioctylsulfosuccinate (AOT); (D) PLGA 503 ester terminated; (E) PLGA 502H acid terminated.
Scheme 1
Scheme 1
Synthesis of a fluorescent tobramycin derivative.
Figure 2
Figure 2
Organic extraction of the fluorescent derivative of tobramycin in the presence (left) and absence (right) of AOT: top layer water, bottom layer dichloromethane.
Figure 3
Figure 3
Effect of pH on the extraction of tobramycin into dichloromethane by AOT.
Figure 4
Figure 4
Release profile of PLGA RG502H nanoparticles entrapped with tobramycin loaded AOT reverse micelles at 37 °C in PBS.
Figure 5
Figure 5
MIC analysis. (A) Unloaded nanoparticle control; (B) Free tobramycin; (C) Tobramycin loaded nanoparticles.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Janam R., Gulati A.K., Nath G. Antibiogram and genotyping of Pseudomonas aeruginosa isolated from human, animal, plant, water and soil sources in north India. Southeast Asian J. Trop. Med. Public Health. 2011;42:1477–1488. - PubMed
    1. Bassetti M., Vena A., Croxatto A., Righi E., Guery B. How to manage Pseudomonas aeruginosa infections. Drugs Context. 2018;7:212527. doi: 10.7573/dic.212527. - DOI - PMC - PubMed
    1. Kassavin D.S., Pascarella L., Goldfarb M.A. Surgical site infections: incidence and trends at a community teaching hospital. Am. J. Surg. 2011;201:749–753. doi: 10.1016/j.amjsurg.2010.03.002. - DOI - PubMed
    1. Mendelson M.H., Gurtman A., Szabo S., Neibart E., Meyers B.R., Policar M., Reddy S. Pseudomonas aeruginosa bacteremia in patients with AIDS. Clin. Infect. Dis. 1994;18:886–895. doi: 10.1093/clinids/18.6.886. - DOI - PubMed
    1. Govan J.R., Deretic V. Microbial pathogenesis in cystic fibrosis: Mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol. Rev. 1996;60:539–574. - PMC - PubMed

LinkOut - more resources