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. 2019 Jun 13;8(6):845.
doi: 10.3390/jcm8060845.

Deregulation of miRNA in Helicobacter pylori-Induced Gastric MALT Lymphoma: From Mice to Human

Alice Blosse  1 , Michael Levy  2   3 , Cyrielle Robe  4 , Cathy Staedel  5 , Christiane Copie-Bergman  6 , Philippe Lehours  7   8
Affiliations

Deregulation of miRNA in Helicobacter pylori-Induced Gastric MALT Lymphoma: From Mice to Human

Alice Blosse et al. J Clin Med. .

Abstract

Gastric MALT lymphoma (GML) is directly caused by Helicobacter pylori infection but occurs only in a small number of infected subjects. Mechanisms underlying the initiation and progression of GML remain unclear. MicroRNAs (miRNAs) are small non-coding RNAs that are now considered as major players in inflammation and carcinogenesis, acting as oncogenes or tumor suppressors. Previous laboratory studies have shown in a GML mouse model that overexpression of a distinct set of five miRNAs (miR-21a, miR-135b, miR-142a, miR-150, miR-155) could play a critical role in the pathogenesis of GML. Our goal was to compare the miRNA expression profile obtained in the GML mouse model to that in human GML (11 cases of GML compared to 17 cases of gastritis control population). RTqPCR on the five dysregulated miRNAs in the GML mouse model and PCR array followed by RTqPCR confirmation showed that four miRNAs were up-regulated (miR-150, miR-155, miR-196a, miR-138) and two miRNAs down-regulated (miR-153, miR-7) in the stomachs of GML patients vs. gastritis control population. The analysis of their validated targets allowed us to postulate that these miRNAs (except miR-138) could act synergistically in a common signaling cascade promoting lymphomagenesis and could be involved in the pathogenesis of GML.

Keywords: Helicobacter; MALT lymphoma; miRNA; proliferation.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Relative expression levels of miR-155, miR-150, miR-21a, miR-135b, and miR-142a in gastritis and gastric mucosa-associated lymphoid tissue (MALT) lymphomas patient stomachs. Expression levels quantified by RT-qPCR for the gastric MALT lymphomas (n = 11) human group was normalized in comparison to expression levels for the gastritis (n = 17) control group. RNU6, RNU5A, and SNORD95 were used to normalize miRNA expression levels. Data are plotted as box plots, with the box representing 50% of values around the median (horizontal line) and the whiskers representing the minimum and maximum of all the data. * p < 0.05.
Figure 2
Figure 2
Relative expression levels of miR-196a-5p, miR-138-5p, miR-7-5p and miR-153-3p in gastritis and gastric MALT lymphomas. Expression levels quantified by RT-qPCR for gastric MALT lymphomas (n = 11) human group was normalized in comparison to expression levels for gastritis (n = 17) control group. RNU6, RNU5A and SNORD95 were used to normalize miRNA expression levels. Data are plotted as box plots, with the box representing 50% of values around the median (horizontal line) and the whiskers representing the minimum and maximum of all the data. * p < 0.05; ** p < 0.01; *** p < 0.001.
Figure 3
Figure 3
miRNA dysregulation in human gastric MALT lymphoma (GML). Schematic illustration of the potential action network of validated targets of miRNAs overexpressed (miR-155, miR-150 and miR-196) or down-regulated (miR-7 and miR-153) in GML patient stomachs.
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