doi: 10.1073/pnas.84.22.7987.
A short 5'-flanking DNA region is sufficient for developmentally correct expression of moth chorion genes in Drosophila
Affiliations
- PMID: 3120180
- PMCID: PMC299461
- DOI: 10.1073/pnas.84.22.7987
Item in Clipboard
A short 5'-flanking DNA region is sufficient for developmentally correct expression of moth chorion genes in Drosophila
Proc Natl Acad Sci U S A.
1987 Nov.
Abstract
Fusions with the bacterial gene for chloramphenicol acetyltransferase followed by P-element-mediated germ-line transformation in Drosophila have permitted localization of the DNA sequence that confers a high degree of developmental specificity on a pair of silkmoth eggshell (chorion) genes. The short, 272-base-pair, 5'-flanking region shared by the divergently transcribed genes is sufficient for developmentally appropriate expression when placed upstream of the chloramphenicol acetyltransferase gene, in either orientation. A highly conserved motif within that region, TCACGT, is essential for chorion-specific expression.
Similar articles
-
Transgenic regulation of moth chorion gene promoters in Drosophila: tissue, temporal, and quantitative control of four bidirectional promoters.J Mol Evol. 1989 Dec;29(6):486-95. doi: 10.1007/BF02602920. J Mol Evol. 1989. PMID: 2559211
-
Regulatory elements controlling chorion gene expression are conserved between flies and moths.Nature. 1985 Oct 3-9;317(6036):453-6. doi: 10.1038/317453a0. Nature. 1985. PMID: 2413366
-
Gene regulation and evolution in the chorion locus of Bombyx mori. Structural and developmental characterization of four eggshell genes and their flanking DNA regions.J Mol Biol. 1986 Jul 5;190(1):23-35. doi: 10.1016/0022-2836(86)90072-0. J Mol Biol. 1986. PMID: 3023635
-
Drosophila chorion genes: cracking the eggshell's secrets.Bioessays. 1991 Mar;13(3):97-105. doi: 10.1002/bies.950130302. Bioessays. 1991. PMID: 1908228 Review.
-
Chorion genes: a landscape of their evolution, structure, and regulation.Annu Rev Entomol. 2015 Jan 7;60:177-94. doi: 10.1146/annurev-ento-010814-020810. Epub 2014 Oct 17. Annu Rev Entomol. 2015. PMID: 25341099 Review.
Cited by
-
Deformed protein binding sites and cofactor binding sites are required for the function of a small segment-specific regulatory element in Drosophila embryos.EMBO J. 1994 May 15;13(10):2362-77. doi: 10.1002/j.1460-2075.1994.tb06520.x. EMBO J. 1994. PMID: 7910795 Free PMC article.
-
Three copies of the early gene 6F6 are interspersed in and around the late chorion gene cluster of Bombyx mori.J Mol Evol. 1992 Apr;34(4):304-14. doi: 10.1007/BF00160237. J Mol Evol. 1992. PMID: 1569584
-
Functional dissection of an early Drosophila chorion gene promoter: expression throughout the follicular epithelium is under spatially composite regulation.EMBO J. 1990 May;9(5):1457-64. doi: 10.1002/j.1460-2075.1990.tb08262.x. EMBO J. 1990. PMID: 2109695 Free PMC article.
-
Tissue-specific expression of silkmoth chorion genes in vivo using Bombyx mori nuclear polyhedrosis virus as a transducing vector.Proc Natl Acad Sci U S A. 1990 May;87(10):3650-4. doi: 10.1073/pnas.87.10.3650. Proc Natl Acad Sci U S A. 1990. PMID: 2187186 Free PMC article.
-
Transgenic regulation of moth chorion gene promoters in Drosophila: tissue, temporal, and quantitative control of four bidirectional promoters.J Mol Evol. 1989 Dec;29(6):486-95. doi: 10.1007/BF02602920. J Mol Evol. 1989. PMID: 2559211
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
