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. 1987 Nov;84(22):7987-91.
doi: 10.1073/pnas.84.22.7987.

A short 5'-flanking DNA region is sufficient for developmentally correct expression of moth chorion genes in Drosophila

S A Mitsialis  1 , N Spoerel, M Leviten, F C Kafatos
Affiliations

A short 5'-flanking DNA region is sufficient for developmentally correct expression of moth chorion genes in Drosophila

S A Mitsialis et al. Proc Natl Acad Sci U S A. 1987 Nov.

Abstract

Fusions with the bacterial gene for chloramphenicol acetyltransferase followed by P-element-mediated germ-line transformation in Drosophila have permitted localization of the DNA sequence that confers a high degree of developmental specificity on a pair of silkmoth eggshell (chorion) genes. The short, 272-base-pair, 5'-flanking region shared by the divergently transcribed genes is sufficient for developmentally appropriate expression when placed upstream of the chloramphenicol acetyltransferase gene, in either orientation. A highly conserved motif within that region, TCACGT, is essential for chorion-specific expression.

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