doi: 10.1126/science.3120311.
Synthesis of a sequence-specific DNA-cleaving peptide
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- PMID: 3120311
- DOI: 10.1126/science.3120311
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Synthesis of a sequence-specific DNA-cleaving peptide
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Abstract
A synthetic 52-residue peptide based on the sequence-specific DNA-binding domain of Hin recombinase (139-190) has been equipped with ethylenediaminetetraacetic acid (EDTA) at the amino terminus. In the presence of Fe(II), this synthetic EDTA-peptide cleaves DNA at Hin recombination sites. The cleavage data reveal that the amino terminus of Hin(139-190) is bound in the minor groove of DNA near the symmetry axis of Hin recombination sites. This work demonstrates the construction of a hybrid peptide combining two functional domains: sequence-specific DNA binding and DNA cleavage.
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