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. 2019 Sep;62(11):713-717.
doi: 10.1002/jlcr.3777. Epub 2019 Aug 1.

Synthesis of carbon-14-labelled peptides

Antonio Dell'isola  1 , Rodney T Brown  1 , Stuart Jones  1 , Sean L Kitson  1 , Thomas S Moody  1 , Jason Syvret  1 , Balasubramaniam Upeandran  1 , William H Watters  1
Affiliations

Synthesis of carbon-14-labelled peptides

Antonio Dell'isola et al. J Labelled Comp Radiopharm. 2019 Sep.

Abstract

Carbon-14 (14 C)-labelled active pharmaceutical ingredients (APIs) and investigational medicinal products (IMPs) are required for phase 0/I to phase III mass balance and micro-dosing clinical trials. In some cases, this may involve the synthesis of 14 C-labelled peptides, and the analysis can be performed by accelerated mass spectrometry (AMS). The 14 C-peptide is typically prepared by the solid-phase peptide synthesis (SPPS) approach using custom-made glassware for the key coupling steps. Further modification of the purified 14 C-peptide can then be performed.

Keywords: accelerated mass spectrometry; biomolecules; carbon-14 peptides; solid-phase peptide synthesis.

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References

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