Plasmodesmata-Related Structural and Functional Proteins: The Long Sought-After Secrets of a Cytoplasmic Channel in Plant Cell Walls

Abstract

Plant cells are separated by cellulose cell walls that impede direct cell-to-cell contact. In order to facilitate intercellular communication, plant cells develop unique cell-wall-spanning structures termed plasmodesmata (PD). PD are membranous channels that link the cytoplasm, plasma membranes, and endoplasmic reticulum of adjacent cells to provide cytoplasmic and membrane continuity for molecular trafficking. PD play important roles for the development and physiology of all plants. The structure and function of PD in the plant cell walls are highly dynamic and tightly regulated. Despite their importance, plasmodesmata are among the few plant cell organelles that remain poorly understood. The molecular properties of PD seem largely elusive or speculative. In this review, we firstly describe the general PD structure and its protein composition. We then discuss the recent progress in identification and characterization of PD-associated plant cell-wall proteins that regulate PD function, with particular emphasis on callose metabolizing and binding proteins, and protein kinases targeted to and around PD.

Keywords: callose; callose synthase; plant cell walls; plasmodesmata.

Figure 1

A simplified schematic representation of plasmodesmata (PD) structural and functional proteins. Permeability of PD in the plant cell wall is controlled by dynamic callose deposition (closed, right side) and degradation (open, left side) at the neck region of PD. ER, endoplasmic reticulum; GSL, glucan synthase-like; PM, plasma membrane; PDCB, PD-associated callose binding protein; PDGB, plasmodesmal associated β-1,3-glucanase; PDLP, PD-localized protein.