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Review
. 2019 Aug;28(8):1412-1422.
doi: 10.1002/pro.3668. Epub 2019 Jul 1.

New tools for recombinant protein production in Escherichia coli: A 5-year update

Germán L Rosano  1 , Enrique S Morales  1 , Eduardo A Ceccarelli  1
Affiliations
Review

New tools for recombinant protein production in Escherichia coli: A 5-year update

Germán L Rosano et al. Protein Sci. 2019 Aug.

Abstract

The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, and cost-effectiveness. As such, its protein production capabilities are continuously being improved. Also, the associated tools (such as plasmids and cultivation conditions) are subject of ongoing research to optimize product yield. In this work, we review the latest advances in recombinant protein production in E. coli.

Keywords: E. coli strains; Escherichia coli; affinity tags; expression plasmid; fusion partners; inclusion bodies; recombinant protein production.

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Figures

Figure 1
Figure 1
Toolkits available for recombinant protein production. The items in the figure are those thoroughly tested over many years of widespread use, so novel discoveries described in the text were not included. MB, membrane.
See this image and copyright information in PMC

References

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