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. 2018 Oct 3;14(9):455-464.
doi: 10.6026/97320630014455. eCollection 2018.

Identification and characterization of cresol degrading Pseudomonas monteilii strain SHY from Soil samples

Shainy Nhattuketty Krishnan  1   2 , Anuraj Nayarisseri  3   4 , Usha Rajamanickam  1
Affiliations

Identification and characterization of cresol degrading Pseudomonas monteilii strain SHY from Soil samples

Shainy Nhattuketty Krishnan et al. Bioinformation. .

Abstract

Cresol is an organic pollutant discharged by pharmaceutical, pesticide, coal and gasification industries causing severe organ failure in humans. Therefore, it is of interest to isolate microbes from contaminated site for degrading cresol. We isolated a strain (CR-13) that survives at 5000ppm cresol with about 80 percent cresol degradation ability. Immobilized cells showed >99 percent degradation at high concentration of cresol. The 16S rRNA sequence (accession number: MF278026) deposited in GenBank was used for phylogenetic tree analysis and the strain was grouped under Pseudomonas monteilii. The isolated cresol degrading strain was subsequently named as Pseudomonas monteilii SHY.

Keywords: 16S rRNA Gene Sequencing; Cresol degrading bacteria; Pseudomonas monteilii.

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Figures

Figure 1
Figure 1
: Pseudomonas monteilii strain. (a) Gram stained smear of the isolate (Gram negative), (b) Pseudomonas monteilii growing on nutrient agar
Figure 2
Figure 2
Growth profile of CR-13 at varying cresol concentration at optimized conditions (pH at 6.8, temperature at 28 degree Celcius and MMM 3 medium) is shown.
Figure 3
Figure 3
Cells immobilized on different matrices showing growth at 1000 and 2000 ppm cresol concentration, respectively is shown
Figure 4
Figure 4
Cells immobilized on different matrices showing remnant cresol concentration, where initial concentrations were at 1000 and 2000 ppm, respectively
Figure 5
Figure 5
Graph depicting the cell growth on agar bead along with degraded cresol at varying temperature
Figure 6
Figure 6
Graph depicting the cell growth on agar bead along with degraded cresol at varying pH
Figure 7
Figure 7
Graph depicting the cell growth on agar bead along with degraded cresol for varying agar bead size
Figure 8
Figure 8
Cell growth on Agar bead as matrix
Figure 9
Figure 9
Cresol degradation by cell immobilized on Agar beads
Figure 10
Figure 10
Cresol degradation by free cells
Figure 11
Figure 11
The immobilization matrices used in the study
Figure 12
Figure 12
Phylogenetic affiliation of Sequence of Pseudomonas monteilii SHYagainst all the other species of Pseudomonas monteilii
Figure 13
Figure 13
RNA Secondary Structure of Pseudomonas monteilii strain SHY elucidated by UNAFOLD
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