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. 2019 Jun 15;29(2):020711.
doi: 10.11613/BM.2019.020711.

Analytical evaluation of the new Seal Autoanalyzer 3 High Resolution for urinary iodine determination

Valentina Vidranski  1   2 , Maja Franceschi  1   2 , Dražena Krilić  1 , Tomislav Jukić  1   2 , Ivan Mihaljević  2   3 , Zvonko Kusić  4
Affiliations

Analytical evaluation of the new Seal Autoanalyzer 3 High Resolution for urinary iodine determination

Valentina Vidranski et al. Biochem Med (Zagreb). .

Erratum in

Abstract

Introduction: The aim of the study was to evaluate the analytical performance of the new colorimetric, automatic analyser, Seal AutoAnalyzer 3 High Resolution (Seal AA3 HR) (Seal Analytical, Wisconsin, USA) for urinary iodine measurement.

Materials and methods: This study included testing of several analytical features of the method involving: imprecision (within-run %CVr, between-run %CVb and total laboratory precision %CVl), measurement uncertainty, carryover, linearity and method comparison, with 70 urine samples including the measuring range (20 - 700 µg/L).

Results: Within-run, %CVb and %CVl of two control levels were 2.03% and 3.04%, 0.51% and 2.61%, and 2.09% and 4.01%, respectively. Carryover effect was less than 1%. The linearity was good in the range of urinary iodine values between 60 and 500 µg/L (R2 = 0.99). Good agreement of urinary iodine values was found between manual technique and Seal AA3 HR, using Passing-Bablok regression (y = 7.84 (- 3.00 to 15.29) + 0.95 (0.90 to 1.00) x) and Blant-Altman test. Cusum test for linearity indicates that there is no significant deviation from linearity (P > 0.1).

Conclusions: The obtained results proved excellent precision, reproducibility and linearity, comparable to the already used, manual method. The New Seal AA3 HR automatic analyser is acceptable for urinary iodine measurement with very good analytical characteristics and can be used for urinary iodine epidemiological studies of the Croatian population.

Keywords: colorimetry; iodine; method evaluation; urine.

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Conflict of interest statement

Potential conflict of interest: None declared.

Figures

Figure 1
Figure 1
Plot of observed and expected urinary iodine values (μg/L) in linearity study. R2 - coefficient of determination.
Figure 2
Figure 2
Passing-Bablok regression plots comparing urinary iodine concentrations (μg/L) of manual technique and analyser Seal AA3 HR. Solid line - regression line. Dashed lines - 95% confidence interval of the regression line. Dotted line - identity line (Y=X).
Figure 3
Figure 3
Bland-Altman plot with representation of the limits of agreement. Solid line - mean difference. Dashed lines (SD) - standard deviation.
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