Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Sep:86:102-107.
doi: 10.1016/j.ijid.2019.06.022. Epub 2019 Jun 22.

Detection of multiple viral sequences in the respiratory tract samples of suspected Middle East respiratory syndrome coronavirus patients in Jakarta, Indonesia 2015-2016

Tri Yuli Setianingsih  1 , Ageng Wiyatno  2 , Teguh Sarry Hartono  1 , Evi Hindawati  1 , Rosamarlina  1 , Aghnianditya Kresno Dewantari  2 , Khin Saw Myint  2 , Vivi Lisdawati  1 , Dodi Safari  3
Affiliations

Detection of multiple viral sequences in the respiratory tract samples of suspected Middle East respiratory syndrome coronavirus patients in Jakarta, Indonesia 2015-2016

Tri Yuli Setianingsih et al. Int J Infect Dis. 2019 Sep.

Abstract

Objectives: The identification and analysis of viral etiological agents from suspected Middle East respiratory syndrome coronavirus (MERS-CoV) cases admitted to Prof. Dr. Sulianti Saroso Infectious Disease Hospital (IDH) using molecular assays.

Methods: Biological samples were collected from 13 hospitalized patients suspected of MERS-CoV infection in Prof. Dr. Sulianti Saroso IDH from July 2015 to December 2016. The majority of patients presented with pneumonia, with symptoms including fever (≥37.5 °C), labored breathing, and cough, and with a history of travel to the Middle East. Viral RNA was isolated and converted to cDNA, which was used as a template for the detection of 12 viral panels using conventional PCR and sequencing.

Results: Viral etiological agents detected in the patients were enterovirus D68, dengue virus type 3, rhinovirus C, human coronavirus 229E, herpes simplex virus type 1, influenza virus H1N1, influenza virus H3N2, human metapneumovirus, and rhinovirus A60.

Conclusions: The sequences of nine viral agents under different taxa were detected in suspected MERS-CoV patients, including influenza virus, paramyxovirus, coronavirus, enterovirus, human metapneumovirus, and herpesvirus.

Keywords: Indonesia; MERS-CoV; PCR; Pneumonia; Viral.

PubMed Disclaimer

References

    1. Ahmed A.E. The predictors of 3- and 30-day mortality in 660 MERS-CoV patients. BMC Infect Dis. 2017;17(September) [Cited 23 May 2018]. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5594447/ - PMC - PubMed
    1. Ahmed A.E. The predictors of 3- and 30-day mortality in 660 MERS-CoV patients. BMC Infect Dis. 2017;17(September) [Cited 23 May 2018]. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5594447/ - PMC - PubMed
    1. Amin M., Bakhtiar A., Subarjo M., Aksono E., Widiyanti P., Shimizu K., et al. Screening for Middle East respiratory syndrome coronavirus among febrile Indonesian Hajj pilgrims: A study on 28,197 returning pilgrims. J Infect Prev. 2018;19(September (5)):236–239. - PMC - PubMed
    1. Annamalay A.A., Jroundi I., Bizzintino J., Khoo S.-K., Zhang G., Lehmann D., et al. Rhinovirus C is associated with wheezing and rhinovirus A is associated with pneumonia in hospitalized children in Morocco. J Med Virol. 2016;(September) - PMC - PubMed
    1. Anthony S.J., Epstein J.H., Murray K.A., Navarrete-Macias I., Zambrana-Torrelio C.M., Solovyov A., et al. A strategy to estimate unknown viral diversity in mammals. mBio. 2013;4(5) [Internet] Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760253/ - PMC - PubMed