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Review
. 2019 Jun 24;10(6):422.
doi: 10.3390/mi10060422.

Research Progress of Near-Infrared Fluorescence Immunoassay

Xiao-Hui Chang  1 , Jie Zhang  2 , Lin-Huan Wu  3 , Yan-Kun Peng  4 , Xiang-Ying Yang  5 , Xiao-Lin Li  6 , Ai-Jin Ma  7 , Jun-Cai Ma  8 , Guang-Quan Chen  9
Affiliations
Review

Research Progress of Near-Infrared Fluorescence Immunoassay

Xiao-Hui Chang et al. Micromachines (Basel). .

Abstract

Near-infrared fluorescence probes (NIFPs) have been widely used in immunoassay, bio-imaging and medical diagnosis. We review the basic principles of near-infrared fluorescence and near-infrared detection technology, and summarize structures, properties and characteristics of NIFPs (i.e., cyanines, xanthenes fluorescent dyes, phthalocyanines, porphyrin derivates, single-walled carbon nanotubes (SWCNTs), quantum dots and rare earth compounds). We next analyze applications of NIFPs in immunoassays, and prospect the application potential of lateral flow assay (LFA) in rapid detection of pathogens. At present, our team intends to establish a new platform that has highly sensitive NIFPs combined with portable and simple immunochromatographic test strips (ICTSs) for rapid detection of food-borne viruses. This will provide technical support for rapid detection on the port.

Keywords: immunoassay; immunochromatographic test strips (ICTSs); near-infrared fluorescence probes (NIFPs); pathogen detection; quantum dots; rare earth compounds.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Basic chemical structure of near infrared fluorescent (NIRF) dyes.
Figure 2
Figure 2
The structure of low-noise excitation fluorescence detection of Vibrio Parahemolyticus immunochromatographic test dipstick. (1: sample pad; 2: conjugate pad; 3: antibody carrying membrane; 4: test line; 5: control line; 6: absorbent pad; 7: Bottom plate.).
See this image and copyright information in PMC

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