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. 2019:2023:287-299.
doi: 10.1007/978-1-4939-9593-6_18.

Growth and Purification of Vaccinia Virus Stocks for MPM Imaging

Glennys V Reynoso  1 , John P Shannon  1 , Jeffrey L Americo  2 , James Gibbs  3 , Heather D Hickman  4
Affiliations

Growth and Purification of Vaccinia Virus Stocks for MPM Imaging

Glennys V Reynoso et al. Methods Mol Biol. 2019.

Abstract

This chapter provides methods for the propagation, purification, and titration of vaccinia virus (VACV) and the highly attenuated strain-modified vaccinia Ankara (MVA). Additionally, we provide information on VACV recombinants we have used for intravital imaging with multiphoton excitation.

Keywords: Modified vaccinia Ankara; Vaccinia virus; Virus purification.

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References

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