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Review
. 2020 Jan;249(1):56-75.
doi: 10.1002/dvdy.79. Epub 2019 Jul 11.

Modeling genetic epilepsies in a dish

Wei Niu  1 , Jack M Parent  1
Affiliations
Free article
Review

Modeling genetic epilepsies in a dish

Wei Niu et al. Dev Dyn. 2020 Jan.
Free article

Abstract

Human pluripotent stem cells (hPSCs), including embryonic and induced pluripotent stem cells, provide a powerful platform for mechanistic studies of disorders of neurodevelopment and neural networks. hPSC models of autism, epilepsy, and other neurological disorders are also advancing the path toward designing and testing precision therapies. The field is evolving rapidly with the addition of genome-editing approaches, expanding protocols for the two-dimensional (2D) differentiation of different neuronal subtypes, and three-dimensional (3D) human brain organoid cultures. However, the application of these techniques to study complex neurological disorders, including the epilepsies, remains a challenge. Here, we review previous work using both 2D and 3D hPSC models of genetic epilepsies, as well as recent advances in the field. We also describe new strategies for applying these technologies to disease modeling of genetic epilepsies, and discuss current challenges and future directions.

Keywords: brain organoid; epilepsy; genome editing; human pluripotent stem cells; neurological disorder; seizure disorder.

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References

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