Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jul;9(7):285.
doi: 10.1007/s13205-019-1812-7. Epub 2019 Jun 25.

Aberrant expression of TNRC6a and miR-21 during myocardial infarction

Liang Li  1 , Qiang Chen  1 , Chao Feng  1 , Yongping Jin  1 , Shudong Xia  1
Affiliations

Aberrant expression of TNRC6a and miR-21 during myocardial infarction

Liang Li et al. 3 Biotech. 2019 Jul.

Abstract

In the present study, aberrant expression of trinucleotide repeat-containing gene 6a (TNRC6a) and miR-21 was noted and documented in rat myocardial infarction. Briefly, Sprague-Dawley rat model was used for the development of myocardial infarction. Experiments such as histological analysis were carried out to confirm the histopathology of the myocardial infarction. The expression profile of TNRC6a and miR-21 was identified by using quantitative real-time PCR. In addition, immunoblotting was performed to validate the expression profile of TNRC6a and phosphatase and tensin homolog (PTEN). The histological analysis confirmed the progress of myocardial infarction in rat model. As the disease progresses, the protein TNRC6a expresses abnormally which in turn up-regulates the miR-21 after 3rd and 5th week of infarction. Interestingly, miR-21 binds with its specific target genes PTEN and thereby degrades the target mRNA; as a result, its expression was down-regulated progressively and paved the development of myocardial infarction. The present study concludes that the aberrant expression of TNRC6a and miR-21 was documented during myocardial infarction. These findings play an important role in the diagnosis as well as pave a way for the development of drug targets for treating myocardial infarction.

Keywords: Immunoblotting; Myocardial infarction; PTEN; TNRC6a; miR-21; qRT-PCR.

PubMed Disclaimer

Conflict of interest statement

Conflict of interestThe authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1
Histological analysis of rat tissue samples after myocardial infarction. a Histology of control rat (sham group) tissue sections. b Histology of rat tissue sections after 1st week of myocardial infarction. c Histology of rat tissue sections after 3rd week of myocardial infarction. d Histology of rat tissue sections after 5th week of myocardial infarction
Fig. 2
Fig. 2
Quantitative RT-PCR analysis of TNRC6a and miR-21. Expression analysis of TNRC6a and miR-21 after 1st, 3rd and 5th week of rat myocardial infarction, respectively
Fig. 3
Fig. 3
Immunoblot analysis of TNRC6a and PTEN. Gene expression profile of TNRC6a and PTEN after 1st, 3rd and 5th week of myocardial infarction
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Bartel DP. MicroRNAs: target recognition and regulatory functions. Cell. 2009;136:215–233. doi: 10.1016/j.cell.2009.01.002. - DOI - PMC - PubMed
    1. Boosani CS, Agrawal DK. PTEN modulators: a patent review. Expert Opin Ther Pat. 2013;23:569–580. doi: 10.1517/13543776.2013.768985. - DOI - PMC - PubMed
    1. Chen C, Ridzon DA, Broomer AJ. Real-time quantification of microRNAs by stem–loop RT–PCR. Nucleic Acids Res. 2005;33:e179. doi: 10.1093/nar/gni178. - DOI - PMC - PubMed
    1. Cheng Y, Tan N, Yang J, Liu X, Cao X, He P. A translational study of circulating cell-free microRNA-1 in acute myocardial infarction. Clin Sci (Lond) 2010;119:87–95. doi: 10.1042/CS20090645. - DOI - PMC - PubMed
    1. Davies N. Treating ST-elevation myocardial infarction. Emerg Nurse. 2016;24:20–25. doi: 10.7748/en.24.3.20.s26. - DOI - PubMed