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Review
. 2020 Feb 17;21(4):451-460.
doi: 10.1002/cbic.201900291. Epub 2019 Sep 26.

In-Cell EPR: Progress towards Structural Studies Inside Cells

Alessio Bonucci  1 , Olivier Ouari  2 , Bruno Guigliarelli  3 , Valérie Belle  3 , Elisabetta Mileo  3
Affiliations
Review

In-Cell EPR: Progress towards Structural Studies Inside Cells

Alessio Bonucci et al. Chembiochem. .

Abstract

Exploring the structure and dynamics of biomolecules in the context of their intracellular environment has become the ultimate challenge for structural biology. As the cellular environment is barely reproducible in vitro, investigation of biomolecules directly inside cells has attracted a growing interest. Among magnetic resonance approaches, site-directed spin labeling (SDSL) coupled to electron paramagnetic resonance (EPR) spectroscopy provides competitive and advantageous features to capture protein structure and dynamics inside cells. To date, several in-cell EPR approaches have been successfully applied to both bacterial and eukaryotic cells. In this review, the major advances of in-cell EPR spectroscopy are summarized, as well as the challenges this approach still poses.

Keywords: EPR spectroscopy; SDSL; in-cell spectroscopy; spin labels; structural biology.

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