Weissella cryptocerci sp. nov., isolated from gut of the insect Cryptocercus kyebangensis

Abstract

A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted. Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286^T (95.9 %), Weissella beninensis 2L24P13^T (95.9 %), Weissella fabalis M75^T (95.7 %) and Weissella fabaria 257^T (95.7 %). The phylogenetic tree indicated that the novel organism formed a cluster with W. ghanensis LMG 24286^T, W. beninensis 2L24P13^T, W. fabalis M75^T and W. fabaria 257^T. The G+C content was 41.1 mol% on the basis of the whole-genome sequence. Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminophospholipids, one unidentified phospholipid and four unidentified lipids. The major cellular fatty acids were C_{18 : 1} ω9c, C_{16 : 0}, C_{14 : 0}, summed feature 8 (C_{18 : 1}ω7c and/or C_{18 : 1}ω6c) and summed feature 8 (C_{16 : 1} ω7c and/or C_{16 : 1} ω6c). The cell-wall peptidoglycan was of A4α type with the interpeptide bridge of Gly-d-Glu. Based on these results, strain 26KH-42^T could be classified as a novel species of the genus Weissella, for which the name Weissellacryptocerci sp. nov. is proposed. The type strain is 26KH-42^T (=KACC 18423^T=NBRC 113066^T).

Keywords: Cryptocercus kyebangensis; Weissella cryptocerci; new taxa; taxonomy.