Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jun 26;10(7):486.
doi: 10.3390/genes10070486.

The Genome of the Steller Sea Lion (Eumetopias jubatus)

Harwood H Kwan  1   2 , Luka Culibrk  1   3 , Gregory A Taylor  1 , Sreeja Leelakumari  1 , Ryan Tan  1 , Shaun D Jackman  1 , Kane Tse  1 , Tina MacLeod  1 , Dean Cheng  1 , Eric Chuah  1 , Heather Kirk  1 , Pawan Pandoh  1 , Rebecca Carlsen  1 , Yongjun Zhao  1 , Andrew J Mungall  1 , Richard Moore  1 , Inanc Birol  1   2 , Marco A Marra  1   2 , David A S Rosen  4   5 , Martin Haulena  5 , Steven J M Jones  6   7   8
Affiliations

The Genome of the Steller Sea Lion (Eumetopias jubatus)

Harwood H Kwan et al. Genes (Basel). .

Abstract

The Steller sea lion is the largest member of the Otariidae family and is found in the coastal waters of the northern Pacific Rim. Here, we present the Steller sea lion genome, determined through DNA sequencing approaches that utilized microfluidic partitioning library construction, as well as nanopore technologies. These methods constructed a highly contiguous assembly with a scaffold N50 length of over 14 megabases, a contig N50 length of over 242 kilobases and a total length of 2.404 gigabases. As a measure of completeness, 95.1% of 4104 highly conserved mammalian genes were found to be complete within the assembly. Further annotation identified 19,668 protein coding genes. The assembled genome sequence and underlying sequence data can be found at the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJNA475770.

Keywords: Eumetopias jubatus; Steller sea lion; genome; marine animal; microfluidic partitioning; nanopore.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
A Jupiter plot illustrating the global genome alignment of the Steller sea lion genome (right) to the California sea lion genome (left). Alignment was accomplished with BWA-MEM. Connections within the circle represent alignment between the two assemblies. California sea lion scaffolds over 10 Mb in length were selected. The longest Steller sea lion scaffolds which sum to the same amount of sequence were also selected. Only alignments over 10 Kb in length are displayed.
See this image and copyright information in PMC

References

    1. Pendleton G.W., Pitcher K.W., Fritz L.W., York A.E., Raum-Suryan K.L., Loughlin T.R., Calkins D.G., Hastings K.K., Gelatt T.S. Survival of Steller sea lions in Alaska: A comparison of increasing and decreasing populations. Can. J. Zool. 2006;84:1163–1172. doi: 10.1139/z06-103. - DOI
    1. Bickham J.W., Patton J.C., Loughlin T.R. High variability for control-region sequences in a marine mammal: Implications for conservation and biogeography of Steller sea lions (Eumetopias jubatus) J. Mammal. 1996;77:95–108. doi: 10.2307/1382712. - DOI
    1. Baker A.R., Matson C.W., Trujillo R.G., Bickham J.W., Loughlin T.R., Burkanov V., Calkins D.G., Wickliffe J.K. Variation of mitochondrial control region sequences of Steller sea lions: The three-stock hypothesis. J. Mammal. 2005;86:1075–1084. doi: 10.1644/04-MAMM-A-113R1.1. - DOI
    1. Perez M.A., Merrick R.L., Loughlin T.R. Eumetopias jubatus. Mamm. Species. 1987;283:1–7. doi: 10.2307/3503908. - DOI
    1. Fiscus C.H., Baines G.A. Food and feeding behavior of Steller and California sea lions. J. Mammal. 1966;47:195–200. doi: 10.2307/1378115. - DOI

Publication types