Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jun 27;12(1):363.
doi: 10.1186/s13104-019-4396-8.

Agr typing of Staphylococcus aureus species isolated from clinical samples in training hospitals of Isfahan and Shahrekord

Saeid Javdan  1 , Tahmine Narimani  2 , Milad Shahini Shams Abadi  3 , Abolfazl Gholipour  4   5
Affiliations

Agr typing of Staphylococcus aureus species isolated from clinical samples in training hospitals of Isfahan and Shahrekord

Saeid Javdan et al. BMC Res Notes. .

Abstract

Objective: As an opportunistic pathogen, Staphylococcus aureus is associated with serious nosocomial infections and growing antimicrobial resistance against beta-lactams among S. aureus strains has become a global challenge. The current study was designed to investigate the presence of agr genes among S. aureus strains recovered from clinical samples in university hospitals of Isfahan and Shahrekord.

Results: A total of 150 S. aureus isolates were screened by Disk diffusion method (DDM) and conventional PCR. The minimum (17.3%) and maximum (46%) antibiotic resistance rates were found in vancomycin and cefoxitin, respectively. The majority of our isolates were classified as agr type I followed by type II, type IV, and type III. The statistical analysis showed a significant correlation between agr type I and antibiotic resistance against cefoxitin and erythromycin (p = 0.04 and p = 0.03, respectively). Based on our findings, the agr typing could be considered an effective approach for molecular tracking of S. aureus infections.

Keywords: Agr type; Antibiotic resistance; Methicillin; Staphylococcus aureus; mecA gene.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Lanes 1 and 2: agr type III, lanes 3 and 4: agr type II, lane 5: agr type I, lane 6: agr type IV, M: 100 bp DNA ladder, C− and C+: negative and positive controls
See this image and copyright information in PMC

References

    1. Lowy FD. Antimicrobial resistance: the example of Staphylococcus aureus. J Clin Investig. 2003;111(9):1265–1273. doi: 10.1172/JCI18535. - DOI - PMC - PubMed
    1. Zouhir A, Taieb M, Lamine MA, Cherif A, Jridi T, Mahjoubi B, et al. ANTISTAPHYBASE: database of antimicrobial peptides (AMPs) and essential oils (EOs) against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus. Arch Microbiol. 2017;199(2):215–222. doi: 10.1007/s00203-016-1293-6. - DOI - PubMed
    1. Medina Cruz D, Mi G, Webster TJ. Synthesis and characterization of biogenic selenium nanoparticles with antimicrobial properties made by Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Pseudomonas aeruginosa. J Biomed Mater Res Part A. 2018;106:1400–1412. doi: 10.1002/jbm.a.36347. - DOI - PubMed
    1. García-Álvarez L, Holden MT, Lindsay H, Webb CR, Brown DF, Curran MD, et al. Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study. Lancet Infect Dis. 2011;11(8):595–603. doi: 10.1016/S1473-3099(11)70126-8. - DOI - PMC - PubMed
    1. Gorak EJ, Yamada SM, Brown JD. Community-acquired methicillin-resistant Staphylococcus aureus in hospitalized adults and children without known risk factors. Clin Infect Dis. 1999;29(4):797–800. doi: 10.1086/520437. - DOI - PubMed