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Review
. 2019 Aug;30(8):1358-1364.
doi: 10.1681/ASN.2019040415. Epub 2019 Jun 28.

Renal-Tubule Epithelial Cell Nomenclature for Single-Cell RNA-Sequencing Studies

Lihe Chen  1 , Jevin Z Clark  1 , Jonathan W Nelson  2 , Brigitte Kaissling  3 , David H Ellison  2 , Mark A Knepper  4
Affiliations
Review

Renal-Tubule Epithelial Cell Nomenclature for Single-Cell RNA-Sequencing Studies

Lihe Chen et al. J Am Soc Nephrol. 2019 Aug.

Erratum in

  • Erratum.
    [No authors listed] [No authors listed] J Am Soc Nephrol. 2019 Dec;30(12):2475. doi: 10.1681/ASN.2019101020. J Am Soc Nephrol. 2019. PMID: 31784497 Free PMC article. No abstract available.
No abstract available

Keywords: Epithelia; kidney; transcriptional profiling.

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Figures

Figure 1.
Figure 1.
Renal tubule cell nomenclature. The scheme shows the connection of both a short-looped nephron and a long-looped nephron to the collecting duct system. The numbers point to different renal tubule segments and the cell types that they contain are listed on the right of the scheme. Dashed lines indicate regional boundaries. The corticomedullary junction is defined by the arcuate arteries (not shown in this diagram). The division between the outer stripe and inner stripe of the outer medulla is defined by the transitions from PSTs to DTLs of the loops of Henle. The junction between the outer medulla and inner medulla is defined by the transitions from the ascending thin limbs (ATL) of Henle of the long-loop nephrons to the MTALs of the loops of Henle. The division between the cortical labyrinth and cortical medullary rays is indicated but not labeled. Drawing is on the basis of the original Renal Commission of the International Union of Physiologic Sciences publication.
Figure 2.
Figure 2.
Distributions of transporters in the renal tubule segments from CTAL to CCD in rabbit, mouse, rat, and human kidney. A uniform DCT epithelium expressing NCC (Slc12a3) is present in rabbit, whereas the DCT in mouse, rat, and human has a transitional region (DCT2) expressing NCC and ENaC subunits (Scnn1a, Scnn1b, and Scnn1g). Thus, in rodents and humans, but not rabbit, the DCT is divided into DCT1 and DCT2. The distribution of ICs (mostly type B ICs expressing pendrin [Slc26a4]) is indicated by large dots. AQP2 (Aqp2), vasopressin-dependent water channel aquaporin 2; ENaC (Scnn1a, Scnn1b, and Scnn1g), amiloride-sensitive epithelial Na channel; G, glomerulus; NKCC2 (Slc12a1), bumetanide-sensitive Na-K-2Cl cotransporter; NCC (Slc12a3), thiazide-sensitive Na-Cl cotransporter; TRPM6 (Trpm6), magnesium channel of distal convoluted tubule, TRPV5 (Trpv5), calcium channel of CNT cells.
Figure 3.
Figure 3.
Cell type and protein mass distribution among mouse renal epithelial cells. (A) The contributions of individual mouse renal epithelial cell types to whole kidney epithelial cell count (given at bottom). (B) The contributions of individual mouse renal epithelial cell types to whole kidney tubule protein mass (given at bottom). A-IC, type A intercalated cell; B-IC, type B intercalated cell; Thin Limbs, thin limbs of the loop of Henle (sum of DTL1, DTL2, DTL3, and ascending thin limbs). The data are from Clark et al.
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References

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