Detection of surface carbohydrates on Pneumocystis carinii by fluorescein-conjugated lectins

Abstract

Lectins react with a wide range of different carbohydrates (Table 1). Even so-called monospecific anti-H(O) lectins from Lotus tetragonolobus, Ulex europaeus, and Anguilla anguilla react not only with the anti-H determinant but also with several fucosylated carbohydrates. Consequently, the type of lectin receptor existing on the surface of Pneumocystis carinii should be determined, because only a carbohydrate analysis can demonstrate the kind of carbohydrates which exist on the cell surface of this parasite. For the purpose of this study we used fluorescent isothiocyanata (FITC)-conjugated lectins. Concanavalin A (Con A) and Maclura pomifera (MPA) agglutinin reacted to P. carinii at low concentrations, and the fluorescence intensity was gradually increased with the concentration of the lectins. With lectins from Bauhinia purpurea (BPA), Dolichos biflorus (DBA), Glycine max (SBA), Griffonia simplicifolia (GS-I, GS-II), and Triticum vulgaris (WGA), fluorescence was emitted at high concentrations, while Arachis hypogaea (PNA) and Ulex europaeus (UEA-I) agglutinins did not show fluorescence. The results suggest that P. carinii has abundant Con A- and MPA-specific carbohydrates on the surface.