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. 2019 May 17;10(6):2883-2888.
doi: 10.1364/BOE.10.002883. eCollection 2019 Jun 1.

Laser-assisted optoporation of cells and tissues - a mini-review

Herbert Schneckenburger  1
Affiliations

Laser-assisted optoporation of cells and tissues - a mini-review

Herbert Schneckenburger. Biomed Opt Express. .

Abstract

Laser microbeam techniques are presented, which permit the introduction of molecules or small particles into living cells. Possible mechanisms - including photochemical, photothermal and opto-mechanical interactions (ablations) - are induced by continuous wave (cw) or pulsed lasers of different wavelength, power, and mode of operation. Laser-assisted optoporation permits the uptake of fluorescent dyes as well as DNA plasmids for cell transfection, and, in addition to its broad application to cultivated cells, may have some clinical potential.

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Conflict of interest statement

The authors declare that there are no conflicts of interest related to this article.

Figures

Fig. 1
Fig. 1
CHO cells (a) during laser irradiation (phase contrast) and (b) after irradiation (interference contrast) (488nm; 1 MW/cm2; 2.5 s; image size: 100 × 100 µm2). The arrow marks the irradiated spot. Cells were kept in F-10 HAM medium with an increased amount of light absorbing phenol red (40 µM). Reproduced from Ref. with modifications.
Fig. 2
Fig. 2
Mechanisms causing laser-assisted optoporation (full lines: predominant range; broken lines: possible range).
See this image and copyright information in PMC

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