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. 2019 Jun 20:7:e7022.
doi: 10.7717/peerj.7022. eCollection 2019.

Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts

James William  1 , Peter John  1 , Muhammad Waseem Mumtaz  2 , Ayoub Rashid Ch  1 , Ahmad Adnan  1 , Hamid Mukhtar  3 , Shahzad Sharif  1 , Syed Ali Raza  1 , Muhammad Tayyab Akhtar  3
Affiliations

Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts

James William et al. PeerJ. .

Abstract

Background: Diabetes mellitus type II (DMT-2) is a widely spread metabolic disorder both in developed and developing countries. The role of oxidative stress is well established in DMT-2 pathogenesis. The synthetic drugs for DMT-2 are associated with serious side complications. Antioxidant and α-glucosidase inhibitory actions of phytochemicals from various plant species are considered as an alternative to synthetic drugs for DMT-2 management. The present study aimed to evaluate the antioxidant activity, α-glucosidase inhibitory potential and phytochemical profiling of Hyophorbe lagenicaulis.

Methods: The total phenolic and flavonoid contents, in vitro antioxidant activity (α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging and phosphomolybdenum method) and α-glucosidase inhibition of ultrasonicated hydroethanolic H. lagenicaulis leaf extracts were determined spectrophotometrically. The results of DPPH assay and α-glucosidase inhibition were reported in terms of IC50 value. The phytochemical profiling was accomplished by UHPLC-Q-TOF/MS/MS technique.

Results and discussion: Findings leaped 60% ethanolic extract as rich fraction regarding total phenolic and flavonoid contents. The 60% ethanolic fraction was a promising source of natural antioxidants and α-glucosidase inhibitory agents as indicated by anti-radical and enzyme inibitory activities. Kaempferol, rutin, hesperetin 5-O-glucoside, kaempferol-coumaroyl-glucoside, luteolin 3-glucoside, Isorhamnetin-3-O-rutinoside, trimethoxyflavone derivatives and citric acid were identified by UHPLC-Q-TOF-MS/MS. These compounds were believed to be responsible for the strong antioxidant and enzyme inhibitory activity of plant extracts. The extensive metabolite profiling of H. lagenicaulis was carried out the first time as never reported previously. The H. lagenicaulis might be an appropriate choice to manage diabetes mellitus in an alternate way. The findings may be further exploited extensively for toxicity evaluation to proceed with functional food development having antidiabetic attributes.

Keywords: Antidiabetic; Antioxidant; Diabetes mellitus; Hyophorbe lagenicaulis; UHPLC-Q-TOF-MS/MS.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1. DPPH radicals scavenging activity in terms of IC-50 value for plant extracts and BHA (a, reference; b–f, ascending order of IC-50).
Figure 2
Figure 2. Antioxidant power assay (ASE/g PE) for determination of antioxidant activity of plant extracts and BHA (a, reference; b–e, descending order).
Figure 3
Figure 3. The IC-50 values for α-glucosidase inhibitory potential of extract fractions and acarbose (a, reference; b–f, ascending order of IC-50).
Figure 4
Figure 4. The IC-50 values for α-amylase inhibitory potential of extract fractions and acarbose (a, reference; b–f, ascending order of IC-50).
Figure 5
Figure 5. Main chromatogram of H. lagenicaulis (UHPLC) indicating the peaks of eluted compounds.
Figure 6
Figure 6. Fragmentation pattern of identified compounds with respective m/z values.
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