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Comment
. 2019 Dec;13(1):308-310.
doi: 10.1080/19336950.2019.1638695.

pHluorin-tagged TRPV1 shines light on capsaicin tachyphylaxis

Francina Agosti  1 , Christophe Altier  1
Affiliations
Comment

pHluorin-tagged TRPV1 shines light on capsaicin tachyphylaxis

Francina Agosti et al. Channels (Austin). 2019 Dec.
No abstract available

Keywords: TRPV1; capsaicin; ion channel trafficking; pHluorin; pain; tachyphylaxis.

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Figures

Figure 1.
Figure 1.
Use and advantage of the TRPV1-pHluorin. (a) Schematic of TRPV1-pHluorinshowing insertion of the pHluorin between the His614 and Lys615 upstream of the pore turret of TRPV1. (b) Intra-organelle pH controls the fluorescence of TRPV1-pHluorin. Acidic pH in the Golgi apparatus, secretory vesicles, and lysosomes decreases the pHluorin fluorescence, whereas neutral pH in the ER and PM increases it. (c) Methodological approaches to examine the trafficking, tissue and cellular distribution of TRPV1 as well as conducting biochemical purification and cell sorting using FACS.
See this image and copyright information in PMC

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