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. 1988 Feb 25;16(4):1371-8.
doi: 10.1093/nar/16.4.1371.

Transcriptional stimulation by CaPO4-DNA precipitates

R Pine  1 , D E Levy, N Reich, J E Darnell Jr
Affiliations
Free PMC article

Transcriptional stimulation by CaPO4-DNA precipitates

R Pine et al. Nucleic Acids Res. .
Free PMC article

Abstract

Genes in human chromosomes that normally require induction by alpha-interferon are activated after calcium phosphate (CaPO4) transfection, but not after DEAE-dextran transfection. The c-fos gene and genes stimulated by gamma-interferon also are affected by CaPO4-DNA precipitates, but the calcium ionophore A23187 stimulates only c-fos among this group. These results suggest caution not only in choosing gene transfer methods, but also in interpreting experiments aimed at understanding the role of second messengers in gene activation.

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