Erratum

Abstract

[This corrects the article DOI: 10.1111/j.1476-5381.2011.01780.x.].

Figure 6

GaQ₃ induces FAS‐mediated apoptosis in p53^−/− cells. (A) FAS silencing, p53 silencing and combined silencing of p53 and FAS reduced the GaQ₃‐induced apoptosis in MCF‐7 cells. Populations in upper right and lower right quadrants constitute apoptotic fraction. Apoptotic fractions in control, GaQ₃‐treated, GaQ₃ + p53 siRNA‐treated, GaQ₃ + FAS siRNA‐treated, GaQ₃ + p53 siRNA + FAS siRNA‐treated and GaQ₃ + NAC + p53 siRNA‐treated cells are 0.19 ± 0.04, 67.11 ± 3.29, 22.05 ± 2.68, 39.04 ± 3.42, 7.15 ± 1.92, 15.09 ± 2.25, respectively (mean ± SD; n = 10; P < 0.05). (B) Apoptosis was assessed by flow‐cytometry in GaQ₃‐treated H1299 cells with FAS silencing and Ca²⁺ quenching. Populations in upper right and lower right quadrants constitute apoptotic fraction. Apoptotic fractions in control, GaQ₃‐treated, GaQ₃ + FAS siRNA‐treated and GaQ₃ + Ca²⁺ quenching + FAS siRNA‐treated are 0.17 ± 0.04, 53.21 ± 6.19, 11.16 ± 3.44 and 8.03 ± 2.02, respectively (mean ± SD; n = 10; P < 0.05). (C) (Panel i) RT‐PCR to show increased mRNA for FAS receptor in MCF‐7, H1299 and PC3 cells treated with GaQ₃ at 0, 6, 12, 18 and 24 h respectively. (Panel ii) Western blot analysis to study expression of FAS protein in MCF‐7, H1299 and PC3 cells treated with GaQ₃ for 0, 6, 12, 18 and 24 h respectively. (Panel iii) Western blot to study the role of p53 protein in FAS upregulation upon p53 silencing in GaQ₃‐treated MCF‐7 cells. (D) ELISA of the cell membrane and the nuclear/cytoplasmic fractions of ROS‐quenched, GaQ₃‐treated MCF‐7 and H1299 cells to assess translocation of FAS to the cell membrane (mean ± SD; n = 8; P < 0.05; significantly greater than untreated MCF‐7 cells or H1299 cells).