. 2021 May;34(10):1586-1597.

doi: 10.1080/14767058.2019.1640676. Epub 2019 Jul 15.

Presence of Chlamydia trachomatis DNA in the amniotic fluid in women with preterm prelabor rupture of membranes

Marian Kacerovsky^{1

2}, Roberto Romero^{3

4

5

6}, Lenka Pliskova⁷, Radka Bolehovska⁷, Helena Hornychova⁸, Adela Matejkova⁸, Hana Vosmikova⁸, Ctirad Andrys⁹, Martina Kolackova⁹, Piotr Laudański¹⁰, Vera Pelantova¹¹, Bo Jacobsson^{12

13}, Ivana Musilova¹

Affiliations

¹ Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
² Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.
³ Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U. S. Department of Health and Human Services, Bethesda, MD, and Detroit, MI, USA.
⁴ Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA.
⁵ Department of Epidemiology & Biostatistics, Michigan State University, East Lansing, MI, USA.
⁶ Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, USA.
⁷ Institute of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
⁸ Fingerland Department of Pathology, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
⁹ Department of Clinical Immunology and Allergy, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Poland.
¹⁰ 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Warsaw, Poland.
¹¹ Department of Infectious Diseases, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
¹² Department of Obstetrics and Gynecology, Sahlgrenska Academy, Gothenburg University, Gothenburg, Sweden.
¹³ Department of Genetics and Bioinformatics, Domain of Health Data and Digitalisation, Institute of Public Health, Oslo, Norway.

PMID: 31272257
PMCID: PMC7062296
DOI: 10.1080/14767058.2019.1640676

Presence of Chlamydia trachomatis DNA in the amniotic fluid in women with preterm prelabor rupture of membranes

Marian Kacerovsky et al. J Matern Fetal Neonatal Med. 2021 May.

. 2021 May;34(10):1586-1597.

doi: 10.1080/14767058.2019.1640676. Epub 2019 Jul 15.

Authors

Affiliations

¹ Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
² Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.
³ Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U. S. Department of Health and Human Services, Bethesda, MD, and Detroit, MI, USA.
⁴ Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA.
⁵ Department of Epidemiology & Biostatistics, Michigan State University, East Lansing, MI, USA.
⁶ Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, USA.
⁷ Institute of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
⁸ Fingerland Department of Pathology, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
⁹ Department of Clinical Immunology and Allergy, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Poland.
¹⁰ 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Warsaw, Poland.
¹¹ Department of Infectious Diseases, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
¹² Department of Obstetrics and Gynecology, Sahlgrenska Academy, Gothenburg University, Gothenburg, Sweden.
¹³ Department of Genetics and Bioinformatics, Domain of Health Data and Digitalisation, Institute of Public Health, Oslo, Norway.

PMID: 31272257
PMCID: PMC7062296
DOI: 10.1080/14767058.2019.1640676

Abstract

Objective: The primary aim of this study was to assess the rate and load of amniotic fluid Chlamydia trachomatis DNA and their associations with intra-amniotic infection and intra-uterine inflammatory complications in women with preterm prelabor rupture of membranes (PPROM). The secondary aim was to assess the short-term morbidity of newborns from PPROM pregnancies complicated by amniotic fluid C. trachomatis DNA.

Methods: A retrospective study of 788 women with singleton pregnancies complicated by PPROM between 24 + 0 and 36 + 6 weeks of gestation was performed. Transabdominal amniocenteses were performed at the time of admission. C. trachomatis DNA in the amniotic fluid was assessed by real-time polymerase chain reaction using a commercial AmpliSens® C. trachomatis/Ureaplasma/Mycoplasma hominis-FRT kit, and the level of Ct DNA was quantified.

Results: Amniotic fluid C. trachomatis DNA complicated 2% (16/788) of the PPROM pregnancies and was present in very low loads (median 57 copies DNA/mL). In addition to amniotic fluid C. trachomatis DNA, other bacteria were detected in 62% (10/16) of the C. trachomatis DNA-complicated PPROM pregnancies. Amniotic fluid C. trachomatis DNA was associated with intra-amniotic infection, histologic chorioamnionitis (HCA), and funisitis in 31%, 47%, and 33%, respectively. The presence of C. trachomatis DNA accompanied by Ureaplasma species in the amniotic fluid was associated with a higher rate of HCA than the presence of amniotic fluid C. trachomatis DNA alone. The composite neonatal morbidity in newborns from PPROM pregnancies with amniotic fluid C. trachomatis DNA was 31%.

Conclusion: The presence of C. trachomatis DNA in the amniotic fluid is a relatively rare condition in PPROM. Amniotic fluid C. trachomatis DNA in PPROM is not related to intensive intra-amniotic and intr-auterine inflammatory responses or adverse short-term neonatal outcomes.

Keywords: Amniotic fluid; intra-amniotic inflammation; intra-cellular bacteria; preterm delivery.

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Conflict of interest statement

Disclosure statement: The authors report no conflicts of interest.

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Presence of Chlamydia trachomatis DNA in the amniotic fluid in women with preterm prelabor rupture of membranes

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Presence of Chlamydia trachomatis DNA in the amniotic fluid in women with preterm prelabor rupture of membranes

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