Effect of oophorosalpingo-hysterectomy on serum antioxidant enzymes in female dogs

Abstract

There are few studies evaluating the oxidant-antioxidant status after oophorosalpingohysterectomy (OSH) in female dogs. Here we determined the effect of OSH on antioxidant enzymes in serum, and quantified morphological changes in subcutaneous adipocytes. Lateral OSH was performed in 12 female dogs. The concentration of 17β-estradiol (17β-E₂), the activities of extracellular superoxide dismutase (SOD-ec), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) were determined. Glutathione (GSH), glutathione disulfide (GSSG), lipid peroxidation (LPO), total antioxidant capacity (TAC), carbonylation and vitamin C were measured in serum. Subcutaneous adipose tissue was obtained to determine morphological changes and cell number, under basal conditions and six months after OSH. The SOD-ec, GPx and GST activities increased significantly (p ≤ 0.05), LPO, carbonylation and GSSG also increased. GSH and vitamin C decreased (p = 0.03). 17β-E₂ tended to decrease six months after OSH. Hypertrophy of subcutaneous adipocytes was observed after OSH from the first month and was accentuated after six months (p = 0.001). The results suggest that 17β-E₂ decreases after OSH and alters the antioxidant enzyme activities in serum thus, redox balance is altered. These changes are associated with an increase in body weight and hypertrophy of subcutaneous adipose tissue.

Figure 1

Representative photomicrographs 100x Exfoliative vaginal cytology basal, first and sixth month respectively. Representative photomicrographs in basal conditions (A), first (B) and sixth (C) month after OSH. The cytologies show basal cells of the anestrus stage; this stage is the time between diestrus and the next proestrus and it’s recommended for the realization of the OSH, (n = 12).

Figure 2

(A) Subcutaneous adipose tissue in basal conditions and six months after OSH. (B) Adipocytes diameter in basal conditions and six months after OSH. Values expressed in mean ± standard error, (n = 12). And representative photomicrographs of subcutaneous adipose tissue in (C) basal conditions and D) six months after OSH.

Figure 3

Body weight and fat in dogs females in basal conditions, first and sixth month after performing the OSH. Basal conditions vs. after six months (*p = 0.04). Values expressed as mean ± standard error (n = 12).

Figure 4

SOD-ec expression in basal conditions, first and sixth month after of the OSH. Values expressed in mean ± se. (n = 12).

Figure 5

(A) Enzymatic activity of the GPx, (B) Enzymatic activity of the GST and (C) Enzymatic activity of the GR The experimental conditions were in basal conditions, first and sixth after OSH. Values expressed in mean ± standard error, (n = 12).

Figure 6

(A) Lipid peroxidation (B) Carbonylation, (C) Total antioxidant capacity and (D) Vitamin C. The experimental conditions were in basal conditions, first and sixth month after OSH. Values expressed in mean ± standard error; (n = 12).

Figure 7

Concentration in serum of the: (A) GSH, (B) GSSG, and (C) GSH/GSSG index in basal, first and sex month after of OSH. Values expressed in mean ± standard error (n = 12).