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. 2019 Jun 12;63(2):175-182.
doi: 10.2478/jvetres-2019-0032. eCollection 2019 Jun.

Polymorphisms in the Bovine Tumour Necrosis Factor Receptor Type Two Gene (TNF-RII) and Cell Subpopulations Naturally Infected with Bovine Leukaemia Virus

Alicja Stachura  1 , Barbara Bojarojć-Nosowicz  1 , Dariusz Kaczmarczyk  2 , Ewa Kaczmarczyk  1
Affiliations

Polymorphisms in the Bovine Tumour Necrosis Factor Receptor Type Two Gene (TNF-RII) and Cell Subpopulations Naturally Infected with Bovine Leukaemia Virus

Alicja Stachura et al. J Vet Res. .

Abstract

Introduction: Numerous mutations in the bovine tumour necrosis factor receptor type two (TNF-RII) gene have been identified, but their biological consequences remain poorly understood. The aim of this study was to determine whether polymorphism in the analysed loci of the bovine TNF-RII gene is linked with the size of cell subpopulations naturally infected with bovine leukaemia virus (BLV) which serve important immune functions in the host.

Material and methods: Samples originated from 78 cows. Polymorphisms in the studied gene were determined by PCR-RFLP and DNA sequencing by capillary electrophoresis. BLV infection was diagnosed by the immunofluorescence (IMF) technique and nested PCR. Cell subpopulations were immunophenotyped with IMF.

Results: Similar and non-significant differences in the average percentages of TNFα±, IgM+TNFα±, and CD11b+TNFα±cells infected with BLV were noted in individuals with various genotypes in the polymorphic sites g.-1646T > G and g. 16534T > C of the TNF-RII gene, and significant differences in the percentages of these subpopulations were observed between selected microsatellite genotypes (g.16512CA(n)).

Conclusion: STR polymorphism and the number of CA dinucleotide repeats in intron 1 of the TNF-RII gene influence the frequency of TNF+, CD11b+TNF+, and IgM+TNF+ subpopulations naturally infected with BLV. Polymorphism in the gene's other two sites do not affect the size of these cell subpopulations.

Keywords: BLV; CD11b+TNFα+p24+ cells; IgM+ TNFα+p24+ cells; cattle; microsatellite DNA.

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Conflict of interest statement

Conflict of Interest Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article.

Figures

Fig. 1
Fig. 1
Lymphocyte infected with BLV; BLV-positive and BLV-negative blood lymphocytes; (1,000×, PE staining); photo B. Bojarojć-Nosowicz
Fig. 2
Fig. 2
Detection of epitopes: IgM (A), viral p24 protein (B), mTNF-alfa (C) and all together (D); photo B. Bojarojć-Nosowicz; A–Lymphocyte IgM+ (single-colour reaction, Cascade blue, 1,000×), B–Lymphocyte p24+ (BLV-positive cell) (single-colour reaction, FITC, 1,000×), C–Lymphocyte expressing mTNFα (single-colour reaction, Texas red, 1,000×), D–Lymphocyte IgM+ p24+TNFα+ (triple-colour reaction: Cascade blue, FITC, Texas red, 1,000×)
See this image and copyright information in PMC

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