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. 2019 Oct;60(10):601-606.
doi: 10.1111/jsap.13044. Epub 2019 Jul 5.

Relationship of serum leptin concentration with pituitary-dependent hyperadrenocorticism and cholestatic disease in dogs

S Lee  1 , O-K Kweon  1 , W H Kim  1
Affiliations

Relationship of serum leptin concentration with pituitary-dependent hyperadrenocorticism and cholestatic disease in dogs

S Lee et al. J Small Anim Pract. 2019 Oct.

Abstract

Objectives: To measure serum leptin concentration in dogs with pituitary-dependent hyperadrenocorticism and varying degrees of cholestatic disease and determine whether serum levels differed between dogs with pituitary-dependent hyperadrenocorticism and those with gall bladder mucocoele.

Materials and methods: Client-owned healthy dogs (n=20), dogs diagnosed with gall bladder mucocoele (n=20) and dogs diagnosed with pituitary-dependent hyperadrenocorticism (n=60) were enrolled. Only dogs of normal body condition score were included. Dogs with pituitary-dependent hyperadrenocorticism were divided into three groups according to the severity of cholestatic disease: normal gall bladder (n=20), cholestasis (n=20) and gall bladder mucocoele (n=20). Serum leptin levels were measured using sandwich enzyme-linked immunosorbent assay.

Results: Serum concentrations of leptin were similar between dogs with gall bladder mucocoele and those with pituitary-dependent hyperadrenocorticism accompanied by gall bladder mucocoele; these concentrations were significantly higher than those in healthy control dogs. In dogs with pituitary-dependent hyperadrenocorticism, circulating leptin concentration significantly increased with the severity of cholestasis: higher in the cholestasis group than the normal gall bladder group and higher in the gall bladder mucocoele group than the cholestasis group.

Clinical significance: Elevated circulating leptin concentration was associated with canine pituitary-dependent hyperadrenocorticism and gall bladder mucocoele. Homeostatic imbalance of leptin concentration might be associated with severity of cholestatic disease in pituitary-dependent hyperadrenocorticism.

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Conflict of interest statement

No conflict of interest has been declared.

Figures

Figure 1
Figure 1
Comparison of serum leptin concentrations among healthy dogs (n=20), dogs with gall bladder mucocoele (GBM, n=20), and dogs with pituitary‐dependent hyperadrenocorticism (PDH) exhibiting GBM (n=20). The horizontal bars indicate the median values. **P<0.01, ***P<0.001 between groups
Figure 2
Figure 2
Comparison of serum leptin concentrations among healthy dogs, and pituitary‐dependent hyperadrenocorticism (PDH) patients (n=60) classified according to the degree of cholestatic disease: normal gall bladder (n=20), cholestasis (n=20) and gall bladder mucocoele (GBM, n=20). The horizontal bars indicate the median values. *P<0.05, ***P<0.001 between groups
Figure 3
Figure 3
Comparison of serum leptin concentrations among healthy dogs (n=20) and dogs with pituitary‐dependent hyperadrenocorticism (PDH, n=60), with or without treatment (non‐treated group, n=33; treated group, n=27). The horizontal bars indicate the median values. *P<0.05, **P<0.01, ***P<0.001 between groups
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