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. 2019 Oct:21:102046.
doi: 10.1016/j.nano.2019.102046. Epub 2019 Jul 3.

Interaction of blood plasma proteins with superhemophobic titania nanotube surfaces

Roberta Maia Sabino  1 , Kirsten Kauk  2 , Sanli Movafaghi  3 , Arun Kota  4 , Ketul C Popat  5
Affiliations

Interaction of blood plasma proteins with superhemophobic titania nanotube surfaces

Roberta Maia Sabino et al. Nanomedicine. 2019 Oct.

Abstract

The need to improve blood biocompatibility of medical devices is urgent. As soon as blood encounters a biomaterial implant, proteins adsorb on its surfaces, often leading to several complications such as thrombosis and failure of the device. Therefore, controlling protein adsorption plays a major role in developing hemocompatible materials. In this study, the interaction of key blood plasma proteins with superhemophobic titania nanotube substrates and the blood clotting responses was investigated. The substrate stability was evaluated and fibrinogen adsorption and thrombin formation from plasma were assessed using ELISA. Whole blood clotting kinetics was also investigated, and Factor XII activation on the substrates was characterized by an in vitro plasma coagulation time assay. The results show that superhemophobic titania nanotubes are stable and considerably decrease surface protein adsorption/Factor XII activation as well as delay the whole blood clotting, and thus can be a promising approach for designing blood contacting medical devices.

Keywords: Factor XII; Fibrinogen; Hemocompatibility; Protein adsorption; Superhemophobic; Titania nanotubes.

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Figures

Figure 1
Figure 1
SEM images for (a) Ti, (b) Ti-FL, (c) Ti-PEG, (d) NT (e) NT-FL and (f) NT-PEG.
Figure 2
Figure 2
Static contact angles of human blood plasma for different substrates. No significant differences in contact angle on Ti-PEG and NT. Significant differences in contact angles for all other substrates (p<0.05).
Figure 3
Figure 3
a XPS survey scans for different substrates. b High resolution C1s scans for different substrates. c XRD scans for different substrates.
Figure 4
Figure 4
a Static contact angles for different substrates after 0, 7, 14, 21, and 28 days of incubation. b High resolution C1s scans for different substrates after 28 days of incubation.
Figure 5
Figure 5
Fibrinogen binding from PRP on different substrates.
Figure 6
Figure 6
TAT generation on different substrates after 2 hrs of incubation in PPP. No significant difference between groups was observed (p<0.05).
Figure 7
Figure 7
a FXIIa titration curve for the plasma showing coagulation times versus concentrations of exogenous FXIIa. The fitted curve corresponds to a least squares fitting of a developed mathematical model [5]. b [FXIIa]eq in plasma calculated from coagulation times of different substrates. Ti is used as control for the statistical analysis (p<0.05).
Figure 8
Figure 8
Free hemoglobin concentration values measured in terms of absorbance for different substrates.
See this image and copyright information in PMC

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