Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jul 6;186(8):515.
doi: 10.1007/s00604-019-3604-5.

Visualization and colorimetric determination of clenbuterol in pork by using magnetic beads modified with aptamer and complementary DNA as capture probes, and G-quadruplex/hemin and DNA antibody on the metal-organic framework MIL-101(Fe) acting as a peroxidase mimic

Yuandong Zhang  1 , Hong-Xia Ren  2 , Yang-Bao Miao  3
Affiliations

Visualization and colorimetric determination of clenbuterol in pork by using magnetic beads modified with aptamer and complementary DNA as capture probes, and G-quadruplex/hemin and DNA antibody on the metal-organic framework MIL-101(Fe) acting as a peroxidase mimic

Yuandong Zhang et al. Mikrochim Acta. .

Abstract

A visualization strategy is described for the detection of clenbuterol (CLB). It is using of antibody against dsDNA and G-quadruplex/hemin labeled on a metal organic framework of type MIL-101(Fe) (G-quadruplex/hemin-anti-DNA/MIL-101) acting as a peroxidase mimetic, and magnetic beads modified with aptamer and complementary DNA (MB/Apt-cDNA) as capture probes. The detection reagent was prepared via the reactions between the double stranded DNA (Apt-cDNA) in capture probes and anti-DNA in peroxidase mimetic. In the presence of CLB, the aptamer on the magnetic beads preferentially binds CLB, and the peroxidase mimetic is released to the supernatant after magnetic separation. The released peroxidase mimetic can catalyze the TMB/H2O2 chromogenic system under mild conditions. This leads to the development of a blue-green coloration whose absorbance is measured at 650 nm. The detection limit is as low as 34 fM of CLB. The method was applied to the determination of CLB in pork samples and gave results that were consistent with data obtained with an ELISA kit. Graphical abstract A visualization strategy is described for the detection of clenbuterol. The selectivity of detection system for clenbuterol is excellent compared with other interferents. The method was applied to the determination of CLB in pork samples.

Keywords: Aptamer, peroxidase mimic; Clenbuterol; Metal–organic framework; Visualization strategy.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Zhang Z, Duan F, He L, Peng D, Yan F, Wang M, Zong W, Jia C (2016) Electrochemical clenbuterol immunosensor based on a gold electrode modified with zinc sulfide quantum dots and polyaniline. Microchim Acta 183(3):1089–1097 - DOI
    1. Shellaiah M, Simon T, Venkatesan P, Sun KW, Ko FH, Wu SP (2018) Nanodiamonds conjugated to gold nanoparticles for colorimetric detection of clenbuterol and chromium (III) in urine. Microchim Acta 185(1):74 - DOI
    1. Ji R, Chen S, Xu W, Qin Z, Qiu JF, Li CR (2018) A voltammetric immunosensor for clenbuterol based on the use of a MoS 2-AuPt nanocomposite. Microchim Acta 185(4):209 - DOI
    1. Nieminen MS, Rämö MP, Viitasalo M, Heikkilä P, Karjalainen J, Mäntysaari M, Heikkila J (1996) Serious cardiovascular side effects of large doses of anabolic steroids in weight lifters. Eur Heart J 17(10):1576–1583 - DOI
    1. Cubria JC, Reguera R, Balana-Fouce R, Ordonez C, Ordonez D (1998) Biochemical pharmacology: polyamine-mediated heart hypertrophy induced by Clenbuterol in the mouse. J Pharm Pharmacol 50(1):91–96 - DOI

Publication types

MeSH terms

LinkOut - more resources