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. 2019 May 15:8:665.
doi: 10.12688/f1000research.19230.2. eCollection 2019.

On the explicit use of experimental images in high resolution cryo-EM refinement

Jacqueline Cherfils  1 , Jorge Navaza  1
Affiliations

On the explicit use of experimental images in high resolution cryo-EM refinement

Jacqueline Cherfils et al. F1000Res. .

Abstract

Single particle cryogenic electron microscopy (cryo-EM) is transforming structural biology by enabling the analysis of difficult macromolecular specimens, such as membrane proteins or large complexes with flexible elements, at near atomic resolution with an accuracy close to that of X-ray crystallography. As the technique continues to improve, it is important to assess and exploit its full potential to produce the most possible reliable atomic models. Here we propose to use the experimental images as the data for refinement and validation, instead of the reconstructed maps as currently used. This procedure, which is in spirit quite similar to that used in X-ray crystallography where the data include experimental phases, should contribute to improve the quality of the cryo-EM atomic models.

Keywords: X-ray crystallography; computational method; cryo-EM; model refinement; structural biology.

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Conflict of interest statement

No competing interests were disclosed.

References

    1. Fernandez-Leiro R, Scheres SH: Unravelling biological macromolecules with cryo-electron microscopy. Nature. 2016;537(7620):339–46. 10.1038/nature19948 - DOI - PMC - PubMed
    1. Lyumkis D: Challenges and opportunities in cryo-EM single-particle analysis. J Biol Chem. 2019;294(13):5181–5197. 10.1074/jbc.REV118.005602 - DOI - PMC - PubMed
    1. Vinothkumar KR, Henderson R: Single particle electron cryomicroscopy: trends, issues and future perspective. Q Rev Biophys. 2016;49:e13. 10.1017/S0033583516000068 - DOI - PubMed
    1. Wlodawer A, Li M, Dauter Z: High-Resolution Cryo-EM Maps and Models: A Crystallographer's Perspective. Structure. 2017;25(10):1589–1597.e1. 10.1016/j.str.2017.07.012 - DOI - PMC - PubMed
    1. Afonine PV, Klaholz BP, Moriarty NW, et al. : New tools for the analysis and validation of cryo-EM maps and atomic models. Acta Crystallogr D Struct Biol. 2018;74(Pt 9):814–840. 10.1107/S2059798318009324 - DOI - PMC - PubMed

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