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. 2020 Nov-Dec;86(6):743-747.
doi: 10.1016/j.bjorl.2019.05.004. Epub 2019 Jun 18.

Micronucleus count in nasal epithelial cells from patients with chronic rhinosinusitis and polyps

Renata Loss Drummond  1 , Cláudia Ramos Rhoden  2 , José Faibes Lubianca Neto  2 , Alan da Silveira Fleck  2 , Rita Carolina Pozzer Krumenauer Padoin  2 , Sérgio Luis Amantéa  2
Affiliations

Micronucleus count in nasal epithelial cells from patients with chronic rhinosinusitis and polyps

Renata Loss Drummond et al. Braz J Otorhinolaryngol. 2020 Nov-Dec.

Abstract
in English, Portuguese

Introduction: Chronic rhinosinusitis with nasal polyps, a prevalent disease affecting around 2% of the world population, is characterized by symptomatic inflammation of the nasal mucosa and impairment of quality of life. Chronic rhinosinusitis with nasal polyps has a multifactorial etiology, involving a dysfunctional host response to environmental factors. Thus, inflammatory models may be useful to shed light on the pathophysiology of this disease. Micronucleus count has been used to screen DNA damage in various tissues.

Objective: To investigate the association between frequency of micronucleus in exfoliated cells from the nasal cavity of patients with chronic rhinosinusitis with nasal polyps and disease severity.

Methods: This cross-sectional study included 21 patients with chronic rhinosinusitis with nasal polyps and 19 controls without disease. None of the participants were smokers.

Results: Mean micronucleus count was 3.690 per 1000 cells (±2.165) in individuals with vs. 1.237 per 1000 cells (±0.806) in controls; (Student's t test = 4.653, p < 0.001). Nasal surgery in the past 5 years and aspirin-exacerbated respiratory disease were not associated with nicronucleus count (p = 0.251).

Conclusion: Micronucleus count seems to be linked to chronic rhinosinusitis with nasal polyps, providing a new perspective for the evaluation of this disorder.

Introdução: A rinossinusite crônica com pólipos nasais, doença prevalente que afeta cerca de 2% da população mundial, é caracterizada por inflamação sintomática da mucosa nasal e comprometimento da qualidade de vida. A rinossinusite crônica com pólipos nasais tem etiologia multifatorial, envolvendo resposta disfuncional do hospedeiro a fatores ambientais. Assim, modelos inflamatórios podem ser úteis para esclarecer a fisiopatologia dessa doença. A contagem de micronúcleos tem sido usada para rastrear danos no DNA em vários tecidos.

Objetivo: Investigar a associação entre a frequência de micronúcleos em células esfoliadas da cavidade nasal de pacientes com rinossinusite crônica com pólipos nasais e a gravidade da doença.

Método: Estudo transversal que incluiu 21 pacientes com rinossinusite crônica com pólipos nasais e 19 controles sem doença. Nenhum dos participantes eram fumantes.

Resultados: A contagem média de micronúcleos foi de 3,690 por 1.000 células (±2,165) nos indivíduos doentes e 1,237 por 1.000 células (±0,806) nos controles (Teste t de Student = 4,653; p < 0,001). A cirurgia nasal nos últimos 5 anos e a doença respiratória exacerbada por aspirina não foram associadas à contagem de micronúcleos (p = 0,251).

Conclusão: A contagem de micronúcleos parece estar ligada à rinossinusite crônica com pólipos nasais, proporcionando uma nova perspectiva para a avaliação dessa doença.

Keywords: Inflamação; Inflammation; Micronucleus tests; Nasal polyps; Pólipos nasais; Sinusite; Sinusitis; Teste micronúcleo.

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Figures

Figure 1
Figure 1
Number of micronuclei (MN) per 1000 cells in nasal exfoliative material of individuals with nasal polyps; (*p < 0.001).
Figure 2
Figure 2
Micronuclei (left arrow) and normal nucleus (right arrow) in oral exfoliated epithelial cells (×400).
See this image and copyright information in PMC

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