. 2019 Aug;22(8):1357-1370.

doi: 10.1038/s41593-019-0429-9. Epub 2019 Jul 8.

A whole-brain map of long-range inputs to GABAergic interneurons in the mouse medial prefrontal cortex

Qingtao Sun^#^{1

2}, Xiangning Li^#^{1

2

3}, Miao Ren^{1

2}, Mengting Zhao^{1

2}, Qiuyuan Zhong^{1

2}, Yuqi Ren⁴, Pan Luo^{1

2}, Hong Ni^{1

2}, Xiaoyu Zhang^{1

2}, Chen Zhang^{1

2}, Jing Yuan^{1

2

3}, Anan Li^{1

2

3

5}, Minmin Luo⁴, Hui Gong^{1

2

3

5}, Qingming Luo^{6

7

8}

Affiliations

¹ Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
² MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China.
³ HUST-Suzhou Institute for Brainsmatics, JITRI Institute for Brainsmatics, Suzhou, China.
⁴ National Institute of Biological Science, Beijing, China.
⁵ CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.
⁶ Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China. qluo@mail.hust.edu.cn.
⁷ MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China. qluo@mail.hust.edu.cn.
⁸ HUST-Suzhou Institute for Brainsmatics, JITRI Institute for Brainsmatics, Suzhou, China. qluo@mail.hust.edu.cn.

^# Contributed equally.

PMID: 31285615
DOI: 10.1038/s41593-019-0429-9

A whole-brain map of long-range inputs to GABAergic interneurons in the mouse medial prefrontal cortex

Qingtao Sun et al. Nat Neurosci. 2019 Aug.

. 2019 Aug;22(8):1357-1370.

doi: 10.1038/s41593-019-0429-9. Epub 2019 Jul 8.

Authors

Affiliations

¹ Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
² MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China.
³ HUST-Suzhou Institute for Brainsmatics, JITRI Institute for Brainsmatics, Suzhou, China.
⁴ National Institute of Biological Science, Beijing, China.
⁵ CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.
⁶ Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China. qluo@mail.hust.edu.cn.
⁷ MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China. qluo@mail.hust.edu.cn.
⁸ HUST-Suzhou Institute for Brainsmatics, JITRI Institute for Brainsmatics, Suzhou, China. qluo@mail.hust.edu.cn.

^# Contributed equally.

PMID: 31285615
DOI: 10.1038/s41593-019-0429-9

Abstract

The medial prefrontal cortex (mPFC) contains populations of GABAergic interneurons that play different roles in cognition and emotion. Their local and long-range inputs are incompletely understood. We used monosynaptic rabies viral tracers in combination with fluorescence micro-optical sectioning tomography to generate a whole-brain atlas of direct long-range inputs to GABAergic interneurons in the mPFC of male mice. We discovered that three subtypes of GABAergic interneurons in two areas of the mPFC are innervated by same upstream areas. Input from subcortical upstream areas includes cholinergic neurons from the basal forebrain and serotonergic neurons (which co-release glutamate) from the raphe nuclei. Reconstruction of single-neuron morphology revealed novel substantia innominata-anteromedial thalamic nucleus-mPFC and striatum-anteromedial thalamic nucleus-mPFC circuits. Based on the projection logic of individual neurons, we classified cortical and hippocampal input neurons into several types. This atlas provides the anatomical foundation for understanding the functional organization of the mPFC.

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A whole-brain map of long-range inputs to GABAergic interneurons in the mouse medial prefrontal cortex

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A whole-brain map of long-range inputs to GABAergic interneurons in the mouse medial prefrontal cortex

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