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. 2019 Jun 19;5(6):e01829.
doi: 10.1016/j.heliyon.2019.e01829. eCollection 2019 Jun.

Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae

J M Vargas  1 , M P Moreno Mochi  1 , J M Nuñez  2 , M Cáceres  3 , S Mochi  3 , R Del Campo Moreno  4 , M A Jure  1
Affiliations

Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae

J M Vargas et al. Heliyon. .

Abstract

Carbapenemase-producing Klebsiella pneumoniae (CRKP) are increasingly reported worldwide being necessary the local epidemiological monitoring. Our aim was to characterize the hypermucoviscous CRKP isolates collected in our hospital during a 6 months period. Carriage of the carbapenemase genes (bla KPC, bla NDM, bla VIM and bla OXA-48), extended spectrum β-lactamases (bla SHV-2, bla CTX-M) and the virulence genes (magA, k2A, rmpA, wabG, uge, allS, entB, ycfM, kpn, wcaG, fimH, mrkD, iutA, iroN, hly and cnf-1) were determined by multiplex-PCR. Genetic relationship among the isolates was performed by PFGE and MLST. A total of 35 isolates were recovered, being the urinary and respiratory tract the most common infection sites (34.2%). The bla KPC-2 gene was present in all the isolates, coexisting with bla CTX-M-2 (45.7%), bla SHV-2 (28.6%), and bla CTX-M-2/bla SHV-2 (14.3%). The capsular serotype K2 corresponded with 68.6% of the isolates. Virulence factors frequency were variable [adhesins (97.1%), siderophores (94.3%) and phagocytosis resistance (wabG 48.5%, uge 80% and ycfM 57.1%)]. A total of 10 STs were identified although 40% of them clustered on ST25-CC65, and 17% to ST17. The incidence of KPC-2-producing K. pneumoniae reported by the hospital was 0.290 per 1000 admissions. In summary we described an epidemic scenario of multidrug resistant hypermucoviscous KPC-2 producing ST25 K. pneumoniae in our institution.

Keywords: Carbapenemase; Epidemiology; Infectious disease; KPC; Klebsiella pneumoniae; Microbiology; Multi-clonal.

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Figures

Fig. 1
Fig. 1
DNA finger printing by PFGE and relation to ST type in KPC-2 Klebsiella pneumoniae strains.
See this image and copyright information in PMC

References

    1. Holt K.E., Wertheim H., Zadoks R.N., Baker S., Whitehouse C.A., Dance D., Jenney A., Connor T.R., Hsu L.Y., Severin J., Brisse S., Cao H., Wilksch J., Gorrie C., Schultz M.B., Edwards D.J., Nguyen K.V., Nguyen T.V., Dao T.T., Mensink M., Minh V.L., Nhu N.T., Schultsz C., Kuntaman K., Newton P.N., Moore C.E., Strugnell R.A., Thomson N.R. Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health. Proc. Natl. Acad. Sci. Unit. States Am. 2015;112(27):E3574–E3581. - PMC - PubMed
    1. Boucher H.W., Talbot G.H., Bradley J.S., Edwards J.E., Gilbert D., Rice L.B., Scheld M., Spellberg B., Bartlett J. Bad bugs, no drugs: no ESKAPE! an update from the Infectious Diseases Society of America. Clin. Infect. Dis. 2009;48(1):1–12. - PubMed
    1. Tzouvelekis L.S., Markogiannakis A., Psichogiou M., Tassios P.T., Daikos G.L. Carbapenemases in Klebsiella pneumoniae and other Enterobacteriaceae: an evolving crisis of global dimensions. Clin. Microbiol. Rev. 2012;25(4):682–707. - PMC - PubMed
    1. Catalán-Nájera J.C., Garza-Ramos U., Barrios- Camacho H. 2017. Hypervirulence and Hypermucoviscosity: Two Different but Complementary Klebsiella Spp. Phenotypes? ISSN: 2150-5594 2150-5608 (Online) Journal Homepage.http://www.tandfonline.com/loi/kvir20 - PMC - PubMed
    1. Podschun R., Ullmann U. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin. Microbiol. Rev. 1998;11(4):589–603. - PMC - PubMed

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