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. 1978;3(4):249-55.
doi: 10.1007/BF00251401.

In vitro interaction between 99Tcm-labeled pyrophosphate, 32P-labeled pyrophosphate and rat tissues

M Rohlin, A Larsson, L Hammarström

In vitro interaction between 99Tcm-labeled pyrophosphate, 32P-labeled pyrophosphate and rat tissues

M Rohlin et al. Eur J Nucl Med. 1978.

Abstract

Whole-body sections from young rats were incubated with solutions containing 32P-labeled pyrophosphate, 99Tcm-labeled pyrophosphate and 99Tcm-pertechnetate, respectively, at pH 7.6 or pH 8.6. After incubation, the sections were used for autoradiography and/or staining in order to visualize tissue affinity for 99 Tcm and areas of pyrophosphatase activity. At pH 7.6, the distribution patterns of 99Tcm-labeled pyrophosphate and 32P-labeled pyrophosphate were similar. When pH was raised to 8.6, 99Tcm-labeled pyrophosphate was degraded before reaching the tissues and their enzymes. The distribution pattern of 99Tcm-labeled pyrophosphate at pH 8.6 was similar to that of 99Tcm-pertechnetate. 32P-labeled pyrophosphate could serve as a substrate for alkaline pyrophosphatase and the enzyme activity was demonstrated autoradiographically.

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References

    1. J Nucl Med. 1976 Sep;17(9):820-5 - PubMed
    1. Acta Radiol Suppl. 1954;118:1-110 - PubMed
    1. Radiology. 1974 Sep;112(3):733-5 - PubMed
    1. Scand J Dent Res. 1976 Mar;84(2):63-75 - PubMed
    1. J Nucl Med. 1975 Jan;16(1):33-9 - PubMed