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. 2019 Jun 16:23:e00352.
doi: 10.1016/j.btre.2019.e00352. eCollection 2019 Sep.

FT-IR profiling reveals differential response of roots and leaves to salt stress in a halophyte Sesuvium portulacastrum (L.) L

G C Nikalje  1   2   3 , Jitendra Kumar  1 , T D Nikam  2 , P Suprasanna  1
Affiliations

FT-IR profiling reveals differential response of roots and leaves to salt stress in a halophyte Sesuvium portulacastrum (L.) L

G C Nikalje et al. Biotechnol Rep (Amst). .

Erratum in

Abstract

In a halophyte, Sesuvium portulacastrum (L.) L., we have applied Fourier Transform InfraRed (FT-IR) spectroscopy to detect the corresponding changes associated with salt-induced physiological changes under long- term treatment with 0, 100 and 500 mM NaCl. FT-IR profiles showed changes in chemical composition and functional groups of proteins, lipids and carbohydrates due to salt treatments, evident as differential FT-IR profiles in both roots and leaves specific to these metabolites. Further, the Principle Component Analysis (PCA) was applied to identify the main sources of variation in FT-IR data due to differential treatment. In PCA, the PC1 showed 85.55% and PC2 showed 18.18% variability in data and confirmed differential response of root and leaves to salt treatment in Sesuvium. The results suggest that FT-IR spectrometry can be used to study stress-induced metabolic changes in plants in relation to their salt tolerance.

Keywords: Fourier Transform Infrared Spectroscopy; Metabolic changes; Salt stress; Sesuvium portulacastrum.

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Figures

Fig. 1
Fig. 1
A) FT-IR spectra (range 4000- 400 cm−1) of leaves under NaCl (0, 150, 500 mM) treatment. B) FT-IR spectra (range 4000-400 cm−1) of roots under NaCl (0, 150, 500  mM) treatment.
Fig. 2
Fig. 2
Biplot of FT-IR spectra of root and leaves of FT-IR spectra (range 4000- 400 cm−1) NaCl (0, 150, 500 mM) treatment.
Fig. 3
Fig. 3
Principal Component Analysis of FT-IR spectra (range 4000- 400 cm−1) of roots and leaves under NaCl (0, 150, 500 mM) treatment.
See this image and copyright information in PMC

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