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. 2019 Jul 10;24(14):2519.
doi: 10.3390/molecules24142519.

Rapid Characterization of Triterpene Saponins from Zornia brasiliensis by HPLC-ESI-MS/MS

Yuri Mangueira Nascimento  1 , Lucas Silva Abreu  1 , Ramon Leal Lima  1 , Vicente Carlos O Costa  1 , José Iranildo Miranda de Melo  2 , Raimundo Braz-Filho  3 , Marcelo Sobral Silva  1 , Josean Fechine Tavares  4
Affiliations

Rapid Characterization of Triterpene Saponins from Zornia brasiliensis by HPLC-ESI-MS/MS

Yuri Mangueira Nascimento et al. Molecules. .

Abstract

Zornia brasiliensis Vogel (Leguminosae) is a species popularly known in Brazil as "urinária", "urinana", and "carrapicho", it is popularly used as a diuretic and in the treatment of venereal diseases. A specific methodology to obtain a saponin-enriched fraction and high-performance liquid chromatography coupled with diode array detection, ion trap mass spectrometry, and TOF-MS (HPLC-DAD-ESI-MS/MS) was applied for the analysis of triterpene saponins. The MS and MS/MS experiments were carried out by ionization in negative mode. Molecular mass and fragmentation data were used to support the structural characterization of the saponins. Based on retention times, high-resolution mass determination and fragmentation, 35 oleanane-triterpene saponins were tentatively identified in Z. brasiliensis.

Keywords: HPLC-DAD-ESI-MS/MS; Zornia brasiliensis; soyasaponins; triterpene saponins.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Base peak chromatogram (BPC) of the concentrated fraction of saponins obtained by ESI-MS/MS in negative mode.
Figure 2
Figure 2
Aglycones of Z. brasiliensis saponins tentatively identified by mass spectrometry.
Figure 3
Figure 3
Proposed fragmentation pathway for compound 27 in negative mode ESI-MS/MS. The pathway extends similarly to that for compounds 28, 30, and 34, generating the fragment ions m/z 441 and m/z 439 attributed to the aglycone olean-12-ene-3β,24-diol.
Figure 4
Figure 4
Soyasaponin II (25) isolated from Z. brasiliensis. (formula image) indicates some key HMBC correlations for compound 25.
See this image and copyright information in PMC

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