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. 2019 May 25:31:100570.
doi: 10.1016/j.nmni.2019.100570. eCollection 2019 Sep.

First molecular identification of two Leptospira species (Leptospira interrogans and Leptospira wolffii) in Bangladesh

M A Aziz  1 , M S Aung  2 , S K Paul  1 , S Ahmed  1 , N Haque  1 , S Roy  1 , M Al Amin  1 , A Paul  1 , M A H Miah  1 , M K Alam  1 , M S Islam  3 , M A Hossain  1 , N Kobayashi  2
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First molecular identification of two Leptospira species (Leptospira interrogans and Leptospira wolffii) in Bangladesh

M A Aziz et al. New Microbes New Infect. .

Abstract

Leptospiral 16S rRNA genes were detected in 13 blood samples from 74 febrile patients in north-central Bangladesh, and their sequences phylogenetically clustered with those of Leptospira interrogans or Leptospira wolffii. Genetic diversity in O-antigen polymerase (wzy) was found in an L. interrogans sample.

Keywords: 16S rRNA; Bangladesh; Leptospira interrogans; Leptospira wolffii; wzy.

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Figures

Fig. 1
Fig. 1
Phylogenetic dendrogram based on partial 16S rRNA gene sequences (70 sequence data, 199–350 nucleotides) of Leptospira constructed by maximum likelihood method using MEGA6 program, following alignment with ClustalW algorithm. The trees were statistically supported by bootstrapping with 1000 replicates, and phylogenetic distances were measured by a Kimura two-parameter model with uniform rates among sites. Samples analysed in the present study are marked with closed circles. Three groups (pathogenic, intermediate, non-pathogenic) are shown on the right. Bootstrap values more than 80% are shown. Scale bar represents the genetic distance, i.e. number of substitutions per site.
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