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. 2019 Oct;8(10):e903.
doi: 10.1002/mbo3.903. Epub 2019 Jul 12.

Antioxidant activity of exo-metabolites produced by Fusarium oxysporum: An endophytic fungus isolated from leaves of Otoba gracilipes

Nelson H Caicedo  1 , Andres F Davalos  2 , Paula A Puente  3 , Ana Y Rodríguez  4 , Paola A Caicedo  2
Affiliations

Antioxidant activity of exo-metabolites produced by Fusarium oxysporum: An endophytic fungus isolated from leaves of Otoba gracilipes

Nelson H Caicedo et al. Microbiologyopen. 2019 Oct.

Abstract

Tropical ecosystems hold an extremely diverse array of endophytic fungi, but their potential use still remains to be explored. In this study, we isolated an endophytic fungus from the leaves of Otoba gracilipes, a medicinal tree from a tropical rainforest in Colombia. Following isolation and cultivation, we evaluated its extracellular crude extract for antioxidant activity. Using traditional and molecular methods (ITS1, NL1 regions), the endophyte was identified as Fusarium oxysporum. Fresh spores from the fungal isolate were inoculated in liquid media (potato dextrose broth [PDB] and potato dextrose-yeast extract broth [PDYB]) and centrifuged for recovering extracellular polysaccharides from the exhausted medium after 30 days of cultivation. Crude extracts were recovered, purified, lyophilized, and evaluated for their ability to inactivate the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The extracts obtained from PDB culture media had a 51.5% of scavenging effect on DPPH after 5 min of reaction compared with the extracts from PDBY (26.4%), which suggests a high antioxidant potential of these fungal extracts. Thus, our results suggest other fungi from tropical ecosystems should be explored as potential sources of novel enzymes and other metabolites with bioactivity.

Keywords: Fusarium oxysporum; Otoba gracilipes; antioxidant activity; crude extract; endophytic fungi; tropical forest.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Fusarium oxysporum isolated from leaves of Otoba gracilipes in (a) PDA media, (b) Sabouraud dextrose agar media, and (c) Otoba gracilipes (https://fm-digital-assets.fieldmuseum.org/176/073/MYRI_otob_grac_col_1760717.jpg)
Figure 2
Figure 2
Conidia of the endophytic fungus Fusarium oxysporum (EBB‐ET‐01). (a) Macroconidia (Ma) and microconidia (Mi). (b) Chlamydospores (Cl)
Figure 3
Figure 3
DPPH radical scavenging activity of crude extract obtained from Fusarium oxysporum strain EBB‐ET‐01 growing in potato dextrose broth (PDB) or potato dextrose broth and 0.5% yeast extract (PDYB) at pH 6.0 and 29°C for 30 days
See this image and copyright information in PMC

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