Molecular characterization of Pseudomonas aeruginosa bacteriophages: identification and characterization of the novel virus B86

Abstract

We have characterized a new phage, B86, of Pseudomonas aeruginosa isolated from nature. It is a temperate, uv-inducible, generalized transducing phage. To determine the relatedness of this phage to other characterized P. aeruginosa phages, DNA homology studies were carried out. P. aeruginosa phages have previously been grouped by immunological cross-reactivity. Our studies confirm this classification by demonstrating that phages of different class share little or no DNA homology. Based on homology studies as well as cross-immunity to superinfection, B86 is related to other class B phages and is most homologous with phage B39. The virion morphology of these two phages is quite different, however, as are the restriction enzyme digestion patterns of their genomes with several restriction enzymes. Wild-type B86 is subject to the host-controlled restriction-modification systems of P. aeruginosa PAO and PAT. Virulent mutants of this phage are not restricted by these same restriction-modification systems.