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. 2020 Jan;88(1):106-112.
doi: 10.1002/prot.25773. Epub 2019 Aug 1.

The structure of the extended E2 DNA-binding domain of the bovine papillomavirus-1

Ludmila Leroy  1 , João Alexandre Ribeiro Gonçalves Barbosa  2 , Gonzalo de Prat-Gay  3 , Igor Polikarpov  4 , Carlos Basílio Pinheiro  1
Affiliations

The structure of the extended E2 DNA-binding domain of the bovine papillomavirus-1

Ludmila Leroy et al. Proteins. 2020 Jan.

Abstract

Bovine papillomavirus proteins were extensively studied as a prototype for the human papillomavirus. Here, the crystal structure of the extended E2 DNA-binding domain of the dominant transcription regulator from the bovine papillomavirus strain 1 is described in the space group P31 21. We found two protein functional dimers packed in the asymmetric unit. This new protein arrangement inside the crystal led to the reduction of the mobility of a previously unobserved loop directly involved in the protein-DNA interaction, which was then modeled for the first time.

Keywords: E2 DNA-binding domain; LI loop; bovine papillomavirus; protein structure.

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References

REFERENCES

    1. De Villiers EM, Fauquet C, Broker TR, Bernard HU, zur Hausen H. Classification of papillomaviruses. Virology. 2004;324(1):17-27. http://www.sciencedirect.com/science/article/pii/S004268220400220X.
    1. Chen EY, Howley PM, Levinson AD, Seeburg PH. The primary structure and genetic organization of the bovine papillomavirus type 1 genome. Nature. 1982;299(5883):529-534.
    1. Münger K, Howley PM. Human papillomavirus immortalization and transformation functions. Virus Res. 2002;89(2):213-228. http://www.sciencedirect.com/science/article/pii/S0168170202001909.
    1. Ferguson MK, Botchan MR. Genetic analysis of the activation domain of bovine papillomavirus protein E2: its role in transcription and replication. J Virol. 1996;70(7):4193-4199.
    1. Li R, Botchan MR. Acidic transcription factors alleviate nucleosome-mediated repression of DNA replication of bovine papillomavirus type 1. Proc Natl Acad Sci. 1994;91(15):7051-7055.

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