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. 1988 Feb;79(2):156-9.
doi: 10.1111/j.1349-7006.1988.tb01571.x.

Effect of culture supernatant of MT-2 cells on human immunodeficiency virus-producing cells, MOLT-4/HIVHTLV-IIIB cells

T Matsuyama  1 , Y Hamamoto, T Yoshida, Y Kido, S Kobayashi, N Kobayashi, N Yamamoto
Affiliations

Effect of culture supernatant of MT-2 cells on human immunodeficiency virus-producing cells, MOLT-4/HIVHTLV-IIIB cells

T Matsuyama et al. Jpn J Cancer Res. 1988 Feb.

Abstract

The effect of culture supernatant of MT-2 cells on human immunodeficiency virus (HIV)-producing cells, MOLT-4/HIVHTLV-IIIB cells, was examined. As compared to the effect on MOLT-4 cells, parent cells not infected with HIV, a selective cytotoxic/cytostatic effect on MOLT-4/HIVHTLV-IIIB cells was observed 4 days after treatment with up to 640-fold-diluted MT-2 supernatant. Furthermore, under similar conditions, a 2- to 6-fold increase in the number of HIV particles was detected in the culture of MOLT-4/HIVHTLV-IIIB cells 6 hr after treatment. Complete blocking of these effects by anti-lymphotoxin monoclonal antibody, but not by anti-tumor necrosis factor antibody, indicates that these effects of MT-2 supernatant on MOLT-4/HIVHTLV-IIIB cells are attributable to a lymphotoxin-related cytotoxic factor.

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References

    1. ) Harada , S. , Koyanagi , Y. and Yamamoto , N.Infection of HTLV‐III/LAV in HTLV‐I‐carrying cells MT‐2 and MT‐4 and application in a plaque assay . Science , 229 , 563 – 566 ( 1985. ). - PubMed
    1. ) Koyanagi , Y. , Harada , S. , Takahashi , M. , Uchino , F. and Yamamoto , N.Selective cytotoxicity of AIDS virus infection towards HTLV‐I‐transformed cell line . Int. J. Cancer , 36 , 445 – 451 ( 1985. ). - PubMed
    1. ) Minowada , J. , Ohnuma , T. and Moore , G. E.Rosette‐forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus‐derived lymphocytes . J. Natl. Cancer Inst. 49 , 891 – 895 ( 1972. ). - PubMed
    1. ) Kikukawa , R. , Koyanagi , Y. , Harada , S. , Kobayashi , N. , Hatanaka , M. and Yamamoto , N.Differential susceptibility to the acquired immunodeficiency syndrome retrovirus in cloned cells of human leukemic T‐cell line Molt‐4 . J. Virol , 57 , 1159 – 1162 ( 1986. ). - PMC - PubMed
    1. ) Miyoshi , I. , Kubonishi , I. , Yoshimoto , S. , Akagi , T. , Ohtsuki , Y. , Shiraishi , Y. , Nagata , K. and Hinuma , Y.Type C virus particles in a cord T‐cell line derived by co‐cultivating normal human cord leukocytes and human leukemic T cells . Nature , 294 , 770 – 771 ( 1981. ). - PubMed

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